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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

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If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Mouse primary brown adipocyte Mouse other primary cell siRNA RNA-Interference K2 Transfection System publication J. M. Olsen et al., J. Cell Biol., Nov 2014; 207: 365 - 374 Link

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent siRNA RNA-Interference K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Human peripheral blood mononuclear cell (PBMC)-derived macrophages Human unknown primary cell adherent siRNA RNA-Interference K2 Transfection System publication T. Troiani et al., British Journal of Cancer, 2020, 122: 1782–1790, doi.org/10.1038/s41416-020-0840-8 Link

Mouse podocytes Mouse urinary system primary cell adherent siRNA RNA-Interference K2 Transfection System publication F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4 Link

Mouse immortalized differentiated podocytes Mouse urinary system cell line adherent siRNA RNA-Interference K2 Transfection System publication J. G. Greiten et al., The FASEB Journal, 2021, 35: e21560, DOI: 10.1096/fj.202001179RR Link

293A

Human embryonic kidney adenovirus producer cell line (derivat of HEK293) Human urinary system cell line adherent siRNA RNA-Interference K2 Transfection System publication H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 Link

A-2780

Human ovarian carcinoma cell line Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System application note

A-2780

Human ovarian carcinoma cell line Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System application note

A-2780

Human ovarian carcinoma cell line Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System publication M. Kullmann, 2016, Rheinische Friedrich-Wilhelms-Universität Bonn Link

A-2780cis

Human ovarian carcinoma cell line Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System publication M. Kullmann, 2016, Rheinische Friedrich-Wilhelms-Universität Bonn Link

A-2780cis

Human ovarian carcinoma cell line Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System application note

A-2780cis

Human ovarian carcinoma cell line Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System application note

A375

Human skin malignant melanoma cell line ATCC CRL-1619 Human skin cell line adherent siRNA RNA-Interference K2 Transfection System publication M. Tufano et al., Front. Cell Dev. Biol., 2021, 9: 718947, doi: 10.3389/fcell.2021.718947 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference K2 Transfection System application note

C2C12

Mouse myoblast cell line ATCC CRL-1772 Mouse muscle cell line adherent siRNA RNA-Interference K2 Transfection System publication H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 Link

D54

Human brain glioblastoma cell line Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

D54

Human brain glioblastoma cell line Human brain cell line adherent siRNA RNA-Interference K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

HeLa

Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent siRNA cotransfection (plasmid/siRNA) K2 Transfection System application note

HeLa

Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System application note

HeLa-229

Human cervix adenocarcinoma cell line (derivat of HeLa) Human genital tract cell line adherent siRNA RNA-Interference K2 Transfection System publication D. Kaul et al., Blood Cells, Molecules and Diseases, 2015, 55: 89-93, doi.org/10.1016/j.bcmd.2015.05.001

hMSC

Human mesenchymal stem cells Human unknown stem cell adherent siRNA RNA-Interference K2 Transfection System publication E. A. Ostrakhovitch et al., Exp. Cell Res., 2019, 385: 111683, doi.org/10.1016/j.yexcr.2019.111683 Link

hPASMC

Human pulmonary artery smooth muscle cells Human respiratory system primary cell adherent siRNA RNA-Interference K2 Transfection System publication Z. Qian et al., Am. J. Physiol. Cell Physiol., July 2017, 313: C380 -C391; doi:10.1152/ajpcell.00061.2017

hPBMC

Human peripheral blood monocyte-derived macrophages Human immune system primary cell siRNA RNA-Interference K2 Transfection System publication D. Pires et al., Scientific Reports, 2016, 6:32247, DOI: 10.1038/srep32247 Link

HT-29

Human colorectal adenocarcinoma cell line ATCC HTB-38 Human digestive organs cell line adherent siRNA RNA-Interference K2 Transfection System publication H. Huang et al., Scientific Reports, 2019; 9: 4954, doi: 10.1038/s41598-019-40848-4 Link

LN-229

Human brain glioblastoma cell line ATCC CRL-2611 Human brain cell line adherent siRNA RNA-Interference K2 Transfection System publication H. Lutz, Dissertation, 2019, Technische Universität Darmstadt Link

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