A number of options are available for conclusive detection of the presence of mycoplasmas in contaminated cell cultures. A commonly but unreliable method is the DAPI staining of cells followed by microscopic analysis. However, PCR based methods give the most reliable results for routine checks. Biontex MycoSPY® and MycoSPY® Master Mix detection kits are rapid, reliable and highly sensitive methods of PCR-based mycoplasma detection in cell cultures.
The MycoSPY® kit contains a complete range of reagents for reliable and sensitive PCR. Each mycoplasma test kit contains Primer Mix, Internal Control, Taq Polymerase and a matched Taq polymerase buffer with nucleotide triphosphates (dNTPs). With some other products, the Taq polymerase must be purchased separately and is thus not matched to the buffer supplied in the kit, potentially impairing the sensitivity of detection results.
If mycoplasmas have been detected in your cell culture, the highly effective antibiotic mixture MycoRAZOR® eliminates the contamination rapidly and efficiently.
1. J. J. Jung et al., Stem Cell Reports, 2014, 2: 592–605, doi.org/10.1016/j.stemcr.2014.03.006
2. Arash Yavari et al., Nature Communications, 2017, 8: 1258, DOI: 10.1038/s41467-017-01342-5
More than a quarter of all animal cell cultures are contaminated with Mycoplasma / Mollicutes. The most commonly found strains in cell cultures with a total probability of 94% are: M. fermentans (47%), M. hyorhinis (19%), M. orale (10%), M. arginini (9%), A. laidlawii (6%) and M. hominis (3%). In addition, the following strains were found with lower probability: M. bovis, M. pneumoniae, M. salivarium and M. synoviae. All of these Mollicutes strains are detected by MycoSPY® in addition to a variety of other strains. The verification of the primer specificity was carried out by BLAST analysis.
List of Mollicutes strains detected with MycoSPY®*
||M. pulmonis |
||M. salivarium |
||M. sualvi |
||M. synoviae |
||M. testudineum |
||M. testudinis |
||M. verecundum |
||M. volis |
||M. yeatsii |
||M. zalophi |
||M. zalophidermidis |
|U. cangenitalium ||U. diversum ||U. gallorale
||U. parvurm |
|U. urealyticum || || || |
|M. chaulicola ||M. florum ||M. photuris
||M. tabanidae |
|M. entomobilium ||M. grammopterae ||M. syrphidae
|S. cantharicola ||S. lineolae
||S. taiwanense ||S. citri |
|S. platyhelix || || || |
|A. laidlawii || || || |
* Verification of primer specificity from MycoSPY® Kit by BLAST analysis