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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Mouse podocytes Mouse urinary system primary cell adherent siRNA RNA-Interference K2 Transfection System publication F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4 Link

Rat primary neonatal ventricular cardiomyocytes Rat muscle primary cell plasmid K2 Transfection System application note

Mouse mixed neuron-glia cerebellar cultures Mouse brain unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System application note

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent plasmid K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Human podocytes Human urinary system unknown adherent miRNA K2 Transfection System application note

Mouse immortalized differentiated podocytes Mouse urinary system cell line adherent siRNA RNA-Interference K2 Transfection System publication J. G. Greiten et al., The FASEB Journal, 2021, 35: e21560, DOI: 10.1096/fj.202001179RR Link

Neurosphere derived neuroprogenitor cells (isolated from newborn mice brains) Mouse brain unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System publication S.Y. Kim et al., J. of Cell Science, 2019, 132, jcs228940, doi:10.1242/jcs.228940 Link

Leishmania donovani promastigotes Protozoan unknown unknown plasmid K2 Transfection System publication I. S. Chauhan et al., Acta Tropica, 2019, 199, 105158, doi.org/10.1016/j.actatropica.2019.105158 Link

Leishmania donovani Protozoan unknown unknown plasmid K2 Transfection System application note

Mouse retinal ganglion cells (from multipotent retinal stem cells) Mouse sensory organs stem cell adherent plasmid K2 Transfection System publication D.-W. Chen et al., Prec. Nanomed., 2018, 1(2):106-123, DOI: 10.29016/180711.1 Link

Human peripheral blood mononuclear cell (PBMC)-derived macrophages Human unknown primary cell adherent siRNA RNA-Interference K2 Transfection System publication T. Troiani et al., British Journal of Cancer, 2020, 122: 1782–1790, doi.org/10.1038/s41416-020-0840-8 Link

Human non-embryonic CD34+multipotent stem cells isolated from umbilical cord blood Human other stem cell adherent plasmid K2 Transfection System publication S. Sharma et al., Cell Biol. Int., 2016, 40: 1366-1371, doi: 10.1002/cbin.10681

Mouse primary brown adipocyte Mouse other primary cell siRNA RNA-Interference K2 Transfection System publication J. M. Olsen et al., J. Cell Biol., Nov 2014; 207: 365 - 374 Link

Mouse (BALB/c mice) organ of Corti explants Mouse sensory organs unknown miRNA K2 Transfection System publication Y. Li et al., Cell Death and Disease, 2016, 7:e2362, doi:10.1038/cddis.2016.246 Link

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent siRNA RNA-Interference K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

1C11

Murine neuroepithelial progenitor cells Murine nervous system unknown adherent plasmid K2 Transfection System application note

293A

Human embryonic kidney adenovirus producer cell line (derivat of HEK293) Human urinary system cell line adherent miRNA K2 Transfection System publication H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 Link

293A

Human embryonic kidney adenovirus producer cell line (derivat of HEK293) Human urinary system cell line adherent siRNA RNA-Interference K2 Transfection System publication H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 Link

293FT

Human embryonic kidney virus producer cell line (derivat of 293T) Human urinary system cell line adherent plasmid K2 Transfection System application note

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid stable transfection K2 Transfection System publication C. I. McCarthy et al., J. Gen. Physiol., 2020, 152, 5: e201912492, doi.org/10.1085/jgp.201912492 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid K2 Transfection System publication C. Stegmann et al., J. of Virology, 2019, 93: 11, e00138-19, doi.org/10.1128/JVI.00138-19 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid cotransfection (plasmid/miRNA) K2 Transfection System publication Z. Cai et al., Exp. & Mol. Med., 2018, 50:45, DOI 10.1038/s12276-018-0068-3 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid K2 Transfection System publication C. D. Zappia et al., Cells, 2021, 10, 3026, doi.org/10.3390/cells10113026 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid stable transfection K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System publication C. Zappia et al., Pharmacol. Res. Perspect, 2019, e00531, DOI: 10.1002/prp2.531 Link

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