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Reference type
  1. publication (37)
Origin of cells
  1. bone (2)
  2. breast (1)
  3. digestive organs (10)
  4. genital tract (8)
  5. respiratory system (2)
  6. skin (2)
  7. urinary system (12)
Nucleic acid / Delivered molecule
  1. plasmid (27)
  2. sgRNA (8)
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  1. Metafectene (7)
  2. Metafectene PRO (23)
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References

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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

SNU-449

 Human hepatocellular carcinoma cell line ATCC CRL-2234 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication R. Han et al., Scientific Reports, 2021, 11: 8626, doi.org/10.1038/s41598- Link

SH-SY5Y

 Human neuroblastoma cell line ATCC CRL-2266 Human bone cell line adherent plasmid CRISPR Metafectene PRO publication F. Longo et al., bioRxiv preprint, 2021, doi.org/10.1101/2021.03.16.435646 Link

SH-SY5Y

 Human neuroblastoma cell line ATCC CRL-2266 Human bone cell line adherent plasmid CRISPR Metafectene PRO publication D. Haslinger, Dissertation, 2018, Johann Wolfgang Goethe-Universität Link

NCI-H1299

 Human lung carcinoma cell line ATCC CRL-5803 Human respiratory system cell line adherent plasmid CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

NCI-H1299

 Human lung carcinoma cell line ATCC CRL-5803 Human respiratory system cell line adherent sgRNA CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

MIA PaCa-2

 Human pancreas carcinoma cell line ATCC CRL-1420 Human digestive organs cell line adherent plasmid CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

MIA PaCa-2

 Human pancreas carcinoma cell line ATCC CRL-1420 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

MDA-MB-231

 Human breast adenocarcinoma cell line ATCC HTB-26 Human breast cell line adherent plasmid CRISPR Metafectene PRO publication B. von Neubeck et al., Int. J. of Cancer, 2018, 143: 2065–2075, DOI: 10.1002/ijc.31607

M14

 Human metastatic melanoma cells Human skin cell line adherent plasmid CRISPR Metafectene SI publication A. Sarma et al., The American J. of Pathology, 2021, 191, 2, doi.org/10.1016/j.ajpath.2020.10.015 Link

Hep-3B

 Human hepatocellular carcinoma cell line ATCC HB-8064 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication R. Han et al., Scientific Reports, 2021, 11: 8626, doi.org/10.1038/s41598- Link

Hep-3B

 Human hepatocellular carcinoma cell line ATCC HB-8064 Human digestive organs cell line adherent plasmid CRISPR Metafectene publication Y.-R. Kim et al., Exp. & Mol. Medicine, 2019, 51 :129, doi.org/10.1038/s12276-019-0340-1 Link

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent CRISPR Metafectene PRO publication S. Kumar et al., Dev. Cell., 2019, 49(1): 130–144.e6., doi:10.1016/j.devcel.2019.01.027. Link

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent plasmid CRISPR Metafectene PRO publication A.J. Jakobi et al., Nature Communications, 2020, 11: 440, doi.org/10.1038/s41467-020-14343-8 Link

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent plasmid CRISPR Metafectene PRO publication Y. P. Abudu et al., J. Cell Biol., 2021, 220(8): e202009092, doi: 10.1083/jcb.202009092 Link

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent plasmid CRISPR Metafectene PRO publication A. J. Jakobi et al., Nature Communications, 2020, 11: 440, doi.org/10.1038/s41467-020-14343-8 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR Metafectene publication A. de Araujo Brasil et al., PNAS, 2019, 116, 51: 25991–26000, doi/10.1073/pnas.1902483116 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR K2 Transfection System publication R. Roman et al., BMC Proceedings, 2020, 14 (Suppl 5): P-112 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent CRISPR Metafectene PRO publication S. Kumar et al., Dev. Cell., 2019, 49(1): 130–144.e6., doi:10.1016/j.devcel.2019.01.027. Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR Metafectene publication J. Ohnheiser et al., Biochim. et Biophys. Acta, 2015, 1853: 1564-1573; doi.org/10.1016/j.bbamcr.2015.03.008 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR Metafectene PRO publication D. P. Reguera et al., Cells, 2020, 9: 443, doi:10.3390/cells9020443 Link

HCT116 p53 -/-

 Human colon adenocarcinoma cell line deficient of p53 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

HCT116 p53 -/-

 Human colon adenocarcinoma cell line deficient of p53 Human digestive organs cell line adherent plasmid CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

HCT116

 Human colon adenocarcinoma cell line ATCC CCL-247 Human digestive organs cell line adherent plasmid CRISPR Metafectene PRO publication J. Galán‐Martínez et al., Mol. Oncology, 2021, doi:10.1002/1878-0261.13085 Link

Flp-In TREx HeLa

 Human cervix adenocarcinoma cell line (derivative of HeLa) Human genital tract cell line adherent plasmid CRISPR Metafectene PRO publication T. M. Nthiga et al., The EMBO J., 39: e103649, DOI 10.15252/embj.2019103649 Link

Flp-in TREx 293

 Human embryonic kidney cell line (Derivat of HEK293) Human urinary system cell line adherent plasmid CRISPR Metafectene PRO publication B. K. Shrestha et al., J. Biol. Chem., 2019,doi/10.1074/jbc.RA119.010068 Link

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