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K2® Transfection System

K2® Transfection System

DNA & RNA transfection kit for mammalian cells
Product Information

Highlights:

  • Suitable for transfection of DNA, mRNA, siRNA und miRNA
  • Suitable for cotransfection of DNA and RNA
  • Especially suitable for human cells
  • In many cases significantly increased transfection efficiency compared to common reagents
  • Decreases the cells´ability to detect nucleic acids

Eukaryotic cells have an innate immune system which makes them able to detect foreign substances such as lipopolysaccharides, bacterial or viral nucleic acids and proteins, and take action to inhibit invasion by potential pathogens. Moreover, the cells announce the presence of harmful substances to neighboring cells via messenger molecules. As a consequence, these neighbors adopt defense modus without even having contact with the pathogens.

The finding that lipofection is also attacked by the defense measures of the innate immune system led us to develop the K2® Transfection System, consisting of the K2® Transfection Reagent and the K2® Multiplier. The K2® Transfection Reagent is based on powerful cationic lipids, while the Multiplier decreases the cells' ability to detect nucleic acids.

We were thus able to significantly increase transfection efficiency in many cases in comparison to traditional lipofection reagents, namely in transfection of DNA and mRNA. The K2® Transfection System is therefore suitable for transient and stable transfection of mammalian cell lines and primary cells with DNA (plasmids, bacmide), RNA (mRNA, miRNA, and siRNA), and modified nucleic acids (e.g. antisense oligonucleotides). Areas of application are particularly the production of proteins, antibodies, and viruses (e.g. adenoviruses, AAV, lentiviruses), cotransfections of different nucleic acids, genome editing (e.g. CRISPR/Cas9, CRISPR/Cpf1) and gene silencing (gene knockdown).

 

 

COMPARATIVE STUDIES

DNA TRANSFECTION

Transfection efficiency of K2® Transfection System with pCMV-Luc compared with a commercial transfection reagent (L2K) and METAFECTENE® PRO under optimized conditions:

Transfection efficiency of K2® Transfection System with pCMV-Luc compared with L2k and METAFECTENE® PRO under optimized conditions

 

Transfection efficiency of K2® Transfection System with pCMV-GFP compared with a commercial transfection reagent (L2k) under optimized conditions:

Microscopy after transfection of A549 cells with K2® Transfection System and L2k

Microscopy after transfection of HeLa cells with K2® Transfection System and L2k

Microscopy after transfection of HT 1080 cells with K2® Transfection System and L2k


 

mRNA TRANSFECTION

 

Transfection efficiency of K2® Transfection System with Luciferase encoding mRNA compared with METAFECTENE® PRO under optimized conditions:

Transfection efficiency of K2® Transfection System with Luciferase encoding mRNA compared with METAFECTENE® PRO under optimized conditions

 

Transfection efficiency of K2® Transfection System with GFP encoding mRNA compared with METAFECTENE® PRO under optimized condition:

Microscopy after transfection of A549 cells with GFP encoding mRNA and K2® Transfection System compared to METAFECTENE® PRO

Microscopy after transfection of HeLa cells with GFP encoding mRNA and K2® Transfection System compared to METAFECTENE® PRO

Microscopy after transfection of HepG2 cells with GFP encoding mRNA and K2® Transfection System compared to METAFECTENE® PRO

 

Product Specification

Application Transfection of mammalian cells with DNA & RNA
Content Lipid formulation / sufficient quantity of K2® Multiplier
Assays 500 up to 1850 (48-well) per 1.5 ml reagent (≙ approx.400-500mg nucleic acid)
Shipping At room temperature
Storage 4°C
Shelf life 1 year (from date of delivery)
Product Group Transfection

T060-0.75

K2® Transfection System
750 μl K2® Transfection reagent
3.5 ml K2® Multiplier
DNA & RNA transfection kit for mammalian cells

€200.00
T060-1.0

K2® Transfection System
1.5 ml K2® Transfection reagent
6.5 ml K2® Multiplier
DNA & RNA transfection kit for mammalian cells

€340.00
T060-2.0

K2® Transfection System
2 x 1.5 ml K2® Transfection reagent
2 x 6.5 ml K2® Multiplier
DNA & RNA transfection kit for mammalian cells

€640.00
T060-5.0

K2® Transfection System
5 x 1.5 ml K2® Transfection reagent
5 x 6.5 ml K2® Multiplier
DNA & RNA transfection kit for mammalian cells

€1,500.00