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Origin of cells
  1. bone (22)
  2. brain (38)
  3. breast (43)
  4. digestive organs (131)
  5. genital tract (109)
  6. immune system (22)
  7. nervous system (2)
  8. respiratory system (60)
  9. sensory organs (6)
  10. skin (82)
  11. urinary system (313)
  12. vasculature (5)
  13. other (9)
  14. unknown (23)
Common cell type
  1. cell line (804)
  2. primary cell (38)
  3. stem cell (5)
  4. unknown (19)
Nucleic acid / Delivered molecule
  1. plasmid (652)
  2. bacmid (1)
  3. oligonucleotide (10)
  4. minicircle (12)
  5. MIDGE (6)
  6. GNP-DNA (1)
  7. mRNA (1)
  8. dsRNA (1)
  9. siRNA (160)
  10. miRNA (12)
  11. sgRNA (2)
  12. viral RNA (1)
  13. protein (1)
  14. iron oxide (5)
Application
  1. stable transfection (57)
  2. RNA-Interference (198)
  3. virusproduction (89)
  4. cotransfection (plasmid/plasmid) (53)
  5. cotransfection (plasmid/siRNA) (21)
  6. cotransfection (plasmid/miRNA) (12)
  7. cotransfection (plasmid/sgRNA) (2)
  8. CRISPR (7)
  9. protein production (1)

References

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Products Transfection
Free Sample Offer

Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Human primary fibroblasts Human unknown primary cell siRNA RNA-Interference Metafectene publication L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997 Link

Human primary skin fibroblasts Human skin primary cell adherent siRNA RNA-Interference Metafectene publication M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1 Link

Human Praeadipocytes Human skin primary cell plasmid Metafectene publication G. Weisinger et al., US9663790B2 Link

Human tenon’s capsule fibroblasts Human sensory organs unknown oligonucleotide Metafectene publication H. Mietz et al., Invest. Ophthalmol. Vis. Sci., May 2003; 44: 4290

Human primary chronic lymphocytic leukemia cells Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

Human keratinocytes isolated from neonatal foreskin Human skin primary cell adherent siRNA RNA-Interference Metafectene publication S. Bruche, PhD Thesis, 2014, Imperial College London Link

Primary human skin fibroblasts Human skin primary cell adherent plasmid Metafectene publication T. Kiel, Dissertation 2012, Julius-Maximilians-Universität Würzburg

Human fibrous dysplasia cells Human unknown unknown plasmid Metafectene publication P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44

Human fetal cortical stem cell cultures Human brain stem cell adherent plasmid Metafectene publication S. Couillard-Despres et al., 2014, US 8,841,430 Link

Human primary fibroblasts (derived from ALS patients) Human unknown cell line adherent siRNA RNA-Interference Metafectene publication M. Milani et al., J. of Neuroinflammation, 2021, 18: 132, doi.org/10.1186/s12974-021-02184-1 Link

184A1

 Human immortalized mammary epithelial cells ATCC CRL-8798 Human breast cell line adherent plasmid Metafectene publication K. Konopka et al., Cell Mol Biol Lett, Jan 2009; 14(1): 70-89

1BR3hTERT

 Human hTERT immortalized fibroblasts Human unknown unknown adherent siRNA RNA-Interference Metafectene publication T. Stiff et al., Hum. Mol. Genet., Apr 2016; 25: 1574 - 1587 Link

22Rv1

 Human prostate epithelial cancer cell line ATCC CRL-2505 Human genital tract cell line adherent plasmid Metafectene publication M. Ansems et al., The Prostate, 2012, 72: 1708-1717, DOI 10.1002/pros.22522

293-EBV

 Human embryonic kidney cell line stably carrying recombinant EBV genome (derivat of HEK293) Human urinary system cell line adherent plasmid virusproduction Metafectene publication H. Nowag et al., EBioMedicine, 2014, 1 :116-125, doi.org/10.1016/j.ebiom.2014.11.007 Link

293-EBV

 Human embryonic kidney cell line stably carrying recombinant EBV genome (derivat of HEK293) Human urinary system cell line adherent plasmid virusproduction Metafectene publication B. Neuhierl et al., J. Virol., May 2009; 83: 4616 - 4623 Link

293-EBV

 Human embryonic kidney cell line stably carrying recombinant EBV genome (derivat of HEK293) Human urinary system cell line plasmid Metafectene publication S. Barth et al., Nucleic Acids Res., Feb 2008; 36: 666 - 675 Link

293-EBV

 Human embryonic kidney cell line stably carrying recombinant EBV genome (derivat of HEK293) Human urinary system cell line adherent plasmid virusproduction Metafectene publication R. Feederle et al., J. Virol., May 2009; 83: 4952 - 4962 Link

293-EBV

 Human embryonic kidney cell line stably carrying recombinant EBV genome (derivat of HEK293) Human urinary system cell line adherent plasmid virusproduction Metafectene publication R. Gonnella et al., Bioch. et Biophys. Acta, 2015, 1853: 1586-1595; doi.org/10.1016/j.bbamcr.2015.03.011 Link

293-EBV

 Human embryonic kidney cell line stably carrying recombinant EBV genome (derivat of HEK293) Human urinary system cell line plasmid Metafectene publication M. Granato et al., J. Virol., Apr 2008; 82: 4042 - 4051 Link

293-EBV

 Human embryonic kidney cell line stably carrying recombinant EBV genome (derivat of HEK293) Human urinary system cell line adherent plasmid virusproduction Metafectene publication R. Feederle et al., J. Virol., Nov 2009; 83: 10877 - 10891 Link

293-TLR3

 Human embryonic kidney cell line stably expressing TLR3 (derivat of HEK293) Human urinary system cell line adherent plasmid RNA-Interference Metafectene publication R. Higgs et al., J. Immunol., Aug 2008; 181: 1780 - 1786 Link

293-TLR4/MD2

 Human embryonic kidney cell line stably expressing TLR4/MD2 (derivat of HEK293) Human urinary system cell line adherent plasmid RNA-Interference Metafectene publication R. Higgs et al., J. Immunol., Aug 2008; 181: 1780 - 1786 Link

293/2089

 Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent plasmid virusproduction Metafectene publication S. Iskra et al., J. Virol., Apr 2010; 84: 3612 - 3623 Link

293/delta BALF4

 Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent plasmid virusproduction Metafectene publication B. Neuhierl et al., J. Virol., May 2009; 83: 4616 - 4623 Link

293/delta BBRF1

 Human embryonic kidney virus producer cell line (derivat of HEK293) Human urinary system cell line adherent plasmid Metafectene publication S. Pavlova et al., J. Virol., Feb 2013; 87: 2011 - 2022 Link

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