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Products Transfection
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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Rat primary hepatocytes (Sprague Dawley rats) Rat digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991 Link

Plutella xylostella larvae (diamondback or cabbage moth) Insect unknown unknown siRNA RNA-Interference Metafectene PRO publication W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938 Link

Human primary fibroblasts Human unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication T. Stiff et al., PLoS Genet., 2013, 9(3): e1003360, doi.org/10.1371/journal.pgen.1003360 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8

Mouse cerebral cortical tissue (astrocytes) of 2-day-old SAMR1 and SAMP8 mice Mouse brain primary cell adherent siRNA Metafectene PRO publication M .J. Alvarez-López et al., J. of Neuroinflammation, 2014, 11:126, doi:10.1186/1742-2094-11-126 Link

Mouse fetal ovaries Mouse genital tract primary cell siRNA RNA-Interference Metafectene PRO publication R. Rosario et al., FASEB J., 2019, 33(12): 14221-14233, doi: 10.1096/fj.201901247R. Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication S. Surendran, PhD Thesis, 2014, Indiana University

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental

Human primary hepatocytes Human digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 Link

Human immortalized lymphocyte cell line (immortalized by EBV) Human immune system cell line suspension siRNA RNA-Interference Metafectene PRO publication M. Pradas-Juni et al., Mol. Endocrinoly, 2014, 28(9): 1558-1570, doi: 10.1210/me.2014-1065 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212

Rat primary hepatocytes Rat digestive organs primary cell adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

Mouse articular chondrocytes Mouse unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., Mol. Pharmacol., Sept 2017, 92:366-374, doi.org/10.1124/mol.117.108878

Plutella xylostella larvae (diamondback or cabbage moth) Insect unknown unknown siRNA in vivo application Metafectene PRO publication W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938 Link

1BR-hTERT

Human hTERT-immortalized skin fibroblasts Human skin cell line siRNA RNA-Interference Metafectene PRO publication C. Kerzendorfer et al., Hum. Mol. Genet., Apr 2010; 19: 1324 - 1334 Link

1BR-hTERT

Human hTERT-immortalized skin fibroblasts Human skin cell line siRNA RNA-Interference Metafectene PRO publication H. Brunton et al., Mol. Cell. Biol., Oct 2011; 31: 4022 - 4035 Link

1BR.3

Human primary fibroblasts Human unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication D. Alcantara, PhD Thesis, 2012, University of Sussex Link

1BR.3

Human primary fibroblasts Human unknown cell line adherent siRNA RNA-Interference Metafectene PRO publication I. Abramowicz ez al., HMG Advance Access, 2016

1BR.3

Human primary fibroblasts Human unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication I. Abramowicz et al., Hum. Mol. Genet., Dec 2016, DOI: 10.1093/hmg/ddw364

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent siRNA RNA-Interference Metafectene PRO publication S. Liu et al., J. Biol. Chem., Jul 2015; 290: 17894 - 17908

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent siRNA RNA-Interference Metafectene PRO publication S. Liu et al., J. Biol. Chem., 2015, 290(29): 17894-17908; DOI 10.1074/jbc.M114.607184 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent siRNA RNA-Interference Metafectene PRO publication R. Domínguez-Kelly et al., J. Cell Biol., Aug 2011; 194: 567 - 579 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent siRNA RNA-Interference Metafectene PRO publication S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication R. Domínguez-Kelly et al., J. Cell Biol., Aug 2011; 194: 567 - 579 Link

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