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Species
  1. Hamster (2)
  2. Human (37)
  3. Mouse (4)
Origin of cells
  1. bone (1)
  2. brain (4)
  3. breast (8)
  4. digestive organs (4)
  5. genital tract (9)
  6. immune system (5)
  7. muscle (1)
  8. respiratory system (1)
  9. sensory organs (1)
  10. skin (2)
  11. urinary system (3)
  12. vasculature (2)
  13. unknown (2)
Common cell type
  1. cell line (38)
  2. primary cell (2)
  3. unknown (3)
Application
  1. in vivo application (1)
Product
  1. Metafectene (14)
  2. Metafectene PRO (3)
  3. DOTAP (26)

References

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Products Transfection
Free Sample Offer

Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein Mouse unknown unknown oligonucleotide in vivo application Metafectene publication S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 Link

Human tenon’s capsule fibroblasts Human sensory organs unknown oligonucleotide Metafectene publication H. Mietz et al., Invest. Ophthalmol. Vis. Sci., May 2003; 44: 4290

293T

 Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent oligonucleotide Metafectene publication A. Ploner et al., RNA, Oct 2009; 15: 1797 - 1804 Link

786-O

 Human renal adenocarcinoma cell line ATCC CRL-1932 Human urinary system cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

A549

 Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent oligonucleotide Metafectene PRO publication F. L. Pennemann et al., Nature Commun., 2021, 12: 7009, doi.org/10.1038/s41467-021-27192-w Link

C2C12

 Mouse myoblast cell line ATCC CRL-1772 Mouse muscle cell line adherent oligonucleotide Metafectene publication K.-A. Cho et al., Cells, 2021, 10(8): 2169, doi: 10.3390/cells10082169 Link

CHO-K1

 Chinese hamster ovary cell line ATCC CCL-61 Hamster genital tract cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

GBM

 Human glioblastoma cells Human brain unknown adherent oligonucleotide DOTAP publication M. Janiszewska et al., Genes & Dev., Sep 2012; 26: 1926 - 1944 Link

HCT116

 Human colon adenocarcinoma cell line ATCC CCL-247 Human digestive organs cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent oligonucleotide DOTAP publication E. Aubets et al., Int. J. Mol. Sci., 2021, 22(18): 10025, doi: 10.3390/ijms221810025 Link

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent oligonucleotide DOTAP publication B. Anayat, Dissertation, 2021, University Konstanz Link

HeLa

 Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent oligonucleotide DOTAP publication M. M. M. Enriquez ez al., PLoSONE13(11):e0206818, doi.org/10.1371/journal.pone.0206818 Link

HepG2

 Human hepatocellular carcinoma cell line ATCC HB-8065 Human digestive organs cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

HT-29

 Human colorectal adenocarcinoma cell line ATCC HTB-38 Human digestive organs cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent oligonucleotide Metafectene publication A. H. Wagner et al., Arterioscler Thromb Vasc Biol, Nov 2009; 29: 1894 - 1901 Link

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

K562

 Human myelogenous leukemia cell line ATCC CCL-243 Human immune system cell line suspension oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

LN-229

 Human brain glioblastoma cell line ATCC CRL-2611 Human brain cell line adherent oligonucleotide Metafectene PRO publication P. Codo et al., Oncotarget, 2014, 5 (17) :7651-7662 Link

LN-308

 Human brain glioblastoma cell line Human brain cell line adherent oligonucleotide Metafectene PRO publication P. Codo et al., Oncotarget, 2014, 5 (17) :7651-7662 Link

M21

 Human melanoma cell line Human skin cell line adherent oligonucleotide DOTAP publication M. M. M. Enriquez ez al., PLoSONE13(11):e0206818, doi.org/10.1371/journal.pone.0206818 Link

MCF-7

 Human breast adenocarcinoma cell line ATCC HTB-22 Human breast cell line adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

MCF-7

 Human breast adenocarcinoma cell line ATCC HTB-22 Human breast cell line adherent oligonucleotide Metafectene publication A. M. Tamaddona et al., Iranian J. of Pharm. Sci., 2011, 11: 7(2): 79-87 Link

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