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References

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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

293L

Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent bacmid Metafectene PRO publication M. E. McDowell et al., J. Virol., Jul 2013; 87: 8038 - 8052 Link

293L

Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent bacmid virusproduction Metafectene PRO publication T. Uppal et al., J. of Virology, 2019, 93, 8 e01983-18, doi.org/10.1128/JVI.01983-18 Link

293L

Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent bacmid stable transfection Metafectene PRO publication P. Dabral et al., J. of Virology, 2019, 93: e02256-18, doi.org/10.1128/JVI.02256-18 Link

293L

Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent bacmid stable transfection Metafectene PRO publication T. Uppal et al., J. of Virology, 2019, 93, 8 e01983-18, doi.org/10.1128/JVI.01983-18 Link

293L

Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent bacmid stable transfection Metafectene PRO publication M. E. McDowell, Dissertation, 2012, University of Nevada Link

293L

Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent bacmid virusproduction Metafectene PRO publication R. C. Strahan et al., PLOSPathogens, 2017, doi.org/10.1371/journal.ppat.1006482 Link

293L

Human embryonic kidney cell line (derivat of HEK293) Human urinary system cell line adherent bacmid stable transfection Metafectene PRO publication P. Dabral, Dissertation, 2019, University of Nevada Link

H-5

Trichoplusia ni insect cell line (cabbage looper) Insect genital tract cell line semi-adherent bacmid virusproduction Insectogene publication A. Sadou, PhD Thesis, 2012, University of Milan and University of Naples Link

HEK293

Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent bacmid virusproduction Metafectene publication D. G. van Zyl, Dissertation, 2018, Ruperto-Carola University of Heidelberg

HeLa

Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent bacmid stable transfection Metafectene PRO publication C. Heissenberger et al., Nucleic Acids Res., 2019, 47, 22: 11807–11825, doi: 10.1093/nar/gkz1043 Link

HFF

Human foreskin fibroblasts Human genital tract primary cell adherent bacmid K2 Transfection System publication K. L. Sampaio et al., Viruses, 2021, 13, 1094, doi.org/10.3390/v13061094 Link

HFF

Human foreskin fibroblasts Human genital tract primary cell adherent bacmid virusproduction K2 Transfection System publication K. Laib Sampaio et al., Journal of General Virology, 2016, 97: 1917-1927, DOI 10.1099/jgv.0.000475 Link

HFF

Human foreskin fibroblasts Human genital tract primary cell adherent bacmid virusproduction K2 Transfection System publication C. Stegmann et al., J. of Virology, 2019, 93: 11, e00138-19, doi.org/10.1128/JVI.00138-19 Link

HFFF-tet

Human fetal foreskin fibroblasts (hTERT-immortalized expressing the tetracycline repressor) Human genital tract cell line adherent bacmid virusproduction K2 Transfection System publication K. Laib Sampaio et al., Journal of General Virology, 2016, 97: 1917-1927, DOI 10.1099/jgv.0.000475 Link

HFFFtet

Human fetal foreskin fibroblasts (hTERT-immortalized expressing the tetracycline repressor) Human genital tract cell line adherent bacmid K2 Transfection System publication N. Weiler et al., Viruses, 2021, 13: 614, doi.org/10.3390/v13040614 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent bacmid stable transfection Metafectene publication U. Rand et al., Mol. Systems Biology 8, 2012, 584, doi:10.1038/msb.2012.17 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent bacmid stable transfection Metafectene publication U. Rand et al., Mol Syst Biol, Jul 2014; 8: 584 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent bacmid stable transfection Metafectene PRO publication M. Khateb et al., PLOS ONE, 2016; DOI:10.1371/journal.pone.0156812 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent bacmid Metafectene PRO publication N. Fourier et al., bioRxiv, 2018, doi.org/10.1101/396291 Link

RAW 264.7

Mouse leukaemic monocyte/macrophage cell line ATCC TIB-71 Mouse unknown cell line adherent bacmid stable transfection Metafectene PRO publication M. Khateb et al., PLOS ONE, 2016; DOI:10.1371/journal.pone.0156812 Link

Sf21

Spodoptera frugiperda cell line (army worm) Insect genital tract cell line adherent bacmid virusproduction Insectogene publication G. Male, PhD Thesis, 2014, University of Heidelberg Link

Sf21

Spodoptera frugiperda cell line (army worm) Insect genital tract cell line adherent bacmid virusproduction Insectogene publication M. Zobel et al., J. Cell Biol., 2018, 217 (9): 3161–3182, doi.org/10.1083/jcb.201802023 Link

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