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  2. Remove This Item Species: Rat
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Origin of cells
  1. bone (4)
  2. brain (4)
  3. digestive organs (3)
  4. genital tract (3)
  5. immune system (1)
  6. muscle (6)
  7. nervous system (7)
  8. respiratory system (5)
  9. sensory organs (1)
Nucleic acid / Delivered molecule
  1. plasmid (18)
  2. mRNA (1)
  3. siRNA (11)
  4. miRNA (4)
Application
  1. RNA-Interference (11)
  2. cotransfection (plasmid/siRNA) (2)
  3. 3D cell culture (1)
Product
  1. Metafectene (13)
  2. Metafectene PRO (12)
  3. K2 Transfection System (9)

References

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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

SkM

 Rat primary skeletal myoblasts (from newborn Lewis Rats) Rat bone primary cell adherent plasmid 3D cell culture Metafectene PRO publication B. Blumenthal et al., Artif Organs, Feb 2010; 34(2): E46-54

SkM

 Rat primary skeletal myoblasts (from newborn Lewis Rats) Rat bone primary cell adherent plasmid Metafectene PRO publication B. Blumenthal et al., Eur J Cardiothorac Surg, Oct 2011; 40: e135 - e141 Link

Sertoli

 Rat sertoli cells Rat genital tract primary cell adherent plasmid K2 Transfection System publication H. Chen et al., Endocrinology, 2016, 157(5): 2140 -2159

rTM

 Rat trabecular meshwork cells Rat sensory organs primary cell adherent plasmid Metafectene PRO publication Y. Su et al., Mol. Biol. Rep., 2013, 40: 6091–6096, DOI 10.1007/s11033-013-2720-2

rSkM

 Rat primary sceletal myoblasts Rat muscle primary cell plasmid Metafectene PRO publication R. von Wattenwyl et al., ASAIO Journal, 2012, 58: 268–274, DOI: 10.1097/MAT.0b013e3182523237 Link

rSkM

 Rat primary sceletal myoblasts Rat muscle primary cell plasmid Metafectene PRO publication A. Poppe et al., Artificial Organs, 2012, 36(3): 238–246, doi:10.1111/j.1525-1594.2011.01328.x

rPASMC

 Rat pulmonary artery smooth muscle cells Rat respiratory system primary cell adherent miRNA K2 Transfection System publication J. Chen et al., Physiol Rep., 2017, 5 (18), e13441, https://doi.org/10.14814/phy2.13441 Link

rPASMC

 Rat pulmonary artery smooth muscle cells Rat respiratory system primary cell adherent siRNA RNA-Interference K2 Transfection System publication J. Chen et al., Physiol Rep., 2017, 5 (18), e13441, https://doi.org/10.14814/phy2.13441 Link

rPASMC

 Rat pulmonary artery smooth muscle cells Rat respiratory system primary cell adherent miRNA K2 Transfection System publication J. Chen et al. BMC Genomics, 2016, 17:781, DOI 10.1186/s12864-016-3122-3 Link

rPASMC

 Rat pulmonary artery smooth muscle cells Rat respiratory system primary cell adherent miRNA K2 Transfection System publication Z. Cai et al., Exp. & Mol. Med., 2018, 50:45, DOI 10.1038/s12276-018-0068-3 Link

rPASMC

 Rat pulmonary artery smooth muscle cells Rat respiratory system primary cell adherent miRNA K2 Transfection System publication Z. Qian et al., Oncotarget, 2016, 7 (34):54998 Link

Rat Sertoli cells

 Rat Seroli cells Rat genital tract primary cell adherent plasmid K2 Transfection System publication H. Chen et al., The FASEB Journal, 31 (8): 3587-3607 Link

rat primary astrocytes

 primary astrocytes of cerebral cortices of postnatal day 1 Sprague-Dawley rat Rat brain primary cell adherent plasmid Metafectene PRO publication E.-J. Lee et al., J. of Neuroinflammation, 2018, 15:326, doi.org/10.1186/s12974-018-1363-6 Link

Rat DRG neurons

 Rat primary dorsal root ganglia neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene PRO publication D. S. M. Nascimento et al., Mol. Neurobiol., 2018, 55: 3959–3975, DOI 10.1007/s12035-017-0628-x

Rat DRG neurons

 Rat neurons isolated from the DRG Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2014, DOI 10.1186/s12974-015-0286-8

pBMSC

 Rat primary bone marrow stromal cells Rat bone primary cell adherent plasmid Metafectene publication Z. Jing et al., Hindawi- Stem Cells Int., 2018, Article ID 3069741, doi.org/10.1155/2018/3069741 Link

pBMSC

 Rat primary bone marrow stromal cells Rat bone primary cell adherent plasmid Metafectene publication J. Park et al., Gene Ther. 2003 Jul; 10(13):1089-98

Rat primary hepatocytes (Sprague Dawley rats) Rat digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748 Link

Rat sensory neurons isolated from dorsal root ganglia (DRG) Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529 Link

Rat primary hepatocytes Rat digestive organs primary cell adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent siRNA RNA-Interference K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Rat (Sprague-Dawley) primary alveolar macrophages Rat immune system primary cell plasmid Metafectene publication M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 Link

Primary rat neuronal dendrites Rat nervous system primary cell adherent mRNA Metafectene publication J. Eberwine et al., 2013, US 8,367.402

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