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Species
  1. Chicken (1)
  2. Cow (4)
  3. Human (38)
  4. Mouse (12)
  5. Rabbit (9)
  6. Rat (13)
  7. Swine (3)
Origin of cells
  1. bone (5)
  2. brain (5)
  3. digestive organs (2)
  4. genital tract (5)
  5. immune system (8)
  6. muscle (1)
  7. nervous system (9)
  8. respiratory system (15)
  9. sensory organs (1)
  10. skin (5)
  11. urinary system (1)
  12. vasculature (9)
  13. other (9)
  14. unknown (4)
Nucleic acid / Delivered molecule
  1. plasmid (37)
  2. oligonucleotide (1)
  3. mRNA (1)
  4. siRNA (40)
  5. iron oxide (1)
Application
  1. stable transfection (2)
  2. RNA-Interference (37)
  3. cotransfection (plasmid/plasmid) (4)
  4. cotransfection (plasmid/siRNA) (4)
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References

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Products Transfection
Free Sample Offer

Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Rat DRG neurons

 Rat neurons isolated from the DRG Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2014, DOI 10.1186/s12974-015-0286-8

Rabitt pSkMC

 Rabitt (Lapine) primary skeletal muscle cells Rabbit bone primary cell plasmid Metafectene publication P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44

pPSMC

 Porcine primary smooth muscle cells Swine vasculature primary cell adherent plasmid Metafectene publication J. Pelisek et al., J Mol Med (Berl), Nov 2002; 80(11): 724-36

pPPAEC

 Porcine primary pulmonary artery endothelial cells Swine vasculature primary cell adherent plasmid Metafectene publication B. Wojciak-Stothard et al., Am J Physiol Lung Cell Mol Physiol, Apr 2005; 288: L749 - L760 Link

pHT

 Human primary thyrocytes Human other primary cell plasmid Metafectene publication T. R. H. Büch et al., J. Biol. Chem., Jul 2008; 283: 20330 - 20341 Link

pHEM

 Human primary epidermal melanocytes Human primary cell adherent plasmid Metafectene publication S. Romano et al., Clinical and Translational Medicine, 2014, 3:1, doi:10.1186/2001-1326-3-1 Link

pHDF

 Human primary dermal fibroblasts Human skin primary cell adherent plasmid Metafectene publication L. Atorino et al., J. Cell Biol., Nov 2003; 163: 777 Link

pBMSC

 Rat primary bone marrow stromal cells Rat bone primary cell adherent plasmid Metafectene publication J. Park et al., Gene Ther. 2003 Jul; 10(13):1089-98

pBMSC

 Rat primary bone marrow stromal cells Rat bone primary cell adherent plasmid Metafectene publication Z. Jing et al., Hindawi- Stem Cells Int., 2018, Article ID 3069741, doi.org/10.1155/2018/3069741 Link

pBAEC

 Bovine primary aortic endothelial cells Cow vasculature primary cell adherent siRNA RNA-Interference Metafectene publication Y. Riahi et al., Diabetes, Apr 2010; 59: 808 - 818 Link

pBAEC

 Bovine primary aortic endothelial cells Cow vasculature primary cell adherent plasmid Metafectene publication K. Renner at al., J. Cell. Physiol., 2012, 227: 398–2411, DOI: 10.1002/jcp.22975

PAEC

 Porcine primary aortic endothelial cells Swine vasculature primary cell adherent plasmid Metafectene publication B. Wojciak-Stothard et al., Am J Physiol Lung Cell Mol Physiol, Jun 2006; 290: L1173 - L1182 Link

Mouse DRG neurons

 Mouse primary dorsal root ganglia neurons Mouse nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. X. Chi et al., J. Cell Sci., Dec 2009; 122: 4351 - 4362 Link

Mouse DRG neurons

 Mouse neurons collected from all spinal cord levels of Swiss Webster male mice Mouse nervous system primary cell adherent siRNA RNA-Interference Metafectene publication K. M. Salerno et al., Neuroscience, 2013, 231: 28–37, doi:10.1016/j.neuroscience.2012.11.034. Link

Mouse DRG neurons

 Mouse peripheral dorsal root ganglia neurons Mouse nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. Jing et al., Exp. Neurol., 2012, 233 (1): 221–232, doi:10.1016/j.expneurol.2011.10.005 Link

mDC

 Mouse dentritic cell line Mouse immune system primary cell adherent iron oxide Imaging Metafectene publication E. Küstermann et al., Contrast Media Mol. Imaging, 2008, 3: 27–37

IMR-90

 Human primary lung fibroblasts ATCC CCL-186 Human respiratory system primary cell adherent siRNA RNA-Interference Metafectene publication A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 Link

IMR-90

 Human primary lung fibroblasts ATCC CCL-186 Human respiratory system primary cell adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 Link

IMR-90

 Human primary lung fibroblasts ATCC CCL-186 Human respiratory system primary cell adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 Link

iDC

 Human immature dentritic cells Human immune system primary cell plasmid Metafectene publication Z. K. Nazarkina et al., Mol. Biology, 2018, 52(2): 222–231, DOI: 10.1134/S0026893317050132

hVSMC

 Human primary vascular smooth muscle cells Human vasculature primary cell adherent plasmid stable transfection Metafectene publication G. Weisinger et al., Exp. Cell Res., 2013, 319: 1586-1593, doi.org/10.1016/j.yexcr.2013.04.001

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent oligonucleotide Metafectene publication A. H. Wagner et al., Arterioscler Thromb Vasc Biol, Nov 2009; 29: 1894 - 1901 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent siRNA RNA-Interference Metafectene publication A. Giordano et al., Am J Physiol Heart Circ Physiol, Jan 2012; 302: H135 - H142 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication S. Thakker et al., Oncotarget, 2018, 9 (1): 1210-1228 Link

hTSMC

 Human primary tracheal smooth muscle cells Human respiratory system primary cell adherent siRNA RNA-Interference Metafectene publication C.-W. Lee et al., Mol. Pharmacol., May 2008; 73: 1454 - 1464

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