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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Primary rat neuronal dendrites Rat nervous system primary cell adherent mRNA Metafectene publication J. Eberwine et al., 2013, US 8,367.402

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529 Link

Rat sensory neurons isolated from dorsal root ganglia (DRG) Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748 Link

46C-derived NSCs

Mouse 46C-derived radial glia like neural stem cells Mouse nervous system stem cell adherent plasmid Metafectene EASY publication S. Pachernegg et al., PLOSONE, 2018, doi.org/10.1371/journal.pone.0192242 Link

aNS

Adult Neurospheres (Sphere-forming cells isolated from the lateral walls of the lateral ventricle of C57BL/6 mice) Mouse nervous system unknown siRNA RNA-Interference Metafectene PRO publication B. M. Grebbin et al., Development, Jul 2016; 143: 2281 - 2291 Link

aNS

Adult Neurospheres (Spere-forming cells isolated from lateral walls of lateral ventricle of C57BL/6 mice) Mouse nervous system unknown siRNA 3D cell culture Metafectene PRO publication B. M. Grebbin et al., Development, Jul 2016; 143: 2281 - 2291 Link

D283 Med

Human medulloblastoma cell line ATCC HTB-185 Human nervous system cell line suspension plasmid Metafectene publication S. Couillard-Despres et al., 2014, US 8,841,430 Link

HOG

Human oligodendroglial cells Human nervous system unknown adherent plasmid Metafectene publication G. Chase et al., J. Virol., Jan 2007; 81: 743 - 749 Link

L1–L6 DRG

Mouse lumbar dorsal root ganglia (DRG) Mouse nervous system primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene PRO publication C. Bavassano et al., Biochim. et Biophysica Acta, 2013, 1833: 3166–3175, doi.org/10.1016/j.bbamcr.2013.09.001 Link

Mouse DRG neurons

Mouse primary dorsal root ganglia neurons Mouse nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. X. Chi et al., J. Cell Sci., Dec 2009; 122: 4351 - 4362 Link

Mouse DRG neurons

Mouse primary sensory neurons Mouse nervous system primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene PRO publication C. Bavassano et al., Biochim. et Biophysica Acta, 2013, 1833: 3166–3175, doi.org/10.1016/j.bbamcr.2013.09.001 Link

Mouse DRG neurons

Mouse neurons collected from all spinal cord levels of Swiss Webster male mice Mouse nervous system primary cell adherent siRNA RNA-Interference Metafectene publication K. M. Salerno et al., Neuroscience, 2013, 231: 28–37, doi:10.1016/j.neuroscience.2012.11.034. Link

Mouse DRG neurons

Mouse peripheral dorsal root ganglia neurons Mouse nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. Jing et al., Exp. Neurol., 2012, 233 (1): 221–232, doi:10.1016/j.expneurol.2011.10.005 Link

MSC80

Mouse schwann cell line Mouse nervous system cell line plasmid Metafectene publication V. Phan et al., J. Proteome Res., 2018, 17: 2925−2936, DOI:10.1021/acs.jproteome.8b00022

N1E-115-1

Mouse neuroblastoma cell line (subclone of N1E-115) Mouse nervous system cell line adherent plasmid cotransfection (plasmid/plasmid) Metafectene PRO publication C. Bavassano et al., Biochim. et Biophysica Acta, 2013, 1833: 3166–3175, doi.org/10.1016/j.bbamcr.2013.09.001 Link

N20.1

Mouse embryonic hypothalamus oligidendrocyte cell Line Mouse nervous system cell line adherent plasmid Metafectene publication C. Karl et al., J Neurochemistry, 2005, 92, 264-282

N20.1

Mouse embryonic hypothalamus oligidendrocyte cell Line Mouse nervous system cell line adherent plasmid Metafectene publication S. Couillard-Despres et al., 2014, US 8,841,430 Link

OLN-93

Rat oligodendroglial cells Rat nervous system unknown adherent plasmid stable transfection Metafectene PRO publication K. Pukaß et al., Frontiers in Cellular Neuroscience, 2015, 9: 163; doi: 10.3389/fncel.2015.00163 Link

OLN-t40

Rat oligodendroglial cells (derivat of OLN-93) Rat nervous system unknown adherent plasmid Metafectene PRO publication K. Pukaß et al., J. Neurochem., 2015, 135: 194-205; doi: 10.1111/jnc.13256 Link

OLN-t40

Rat oligodendroglial cells (derivat of OLN-93) Rat nervous system unknown adherent plasmid RNA-Interference Metafectene PRO publication J. Leyket al., J. Mol. Neurosci., 2015, 55:1031-1046; DOI 10.1007/s12031-014-0460-y

OLN-t40

Rat oligodendroglial cells (derivat of OLN-93) Rat nervous system unknown adherent plasmid RNA-Interference Metafectene PRO publication M. Noack et al., GLIA, 2014; 62: 535-547, DOI: 10.1002/glia.22624

OLN-t40

Rat oligodendroglial cells (derivat of OLN-93) Rat nervous system cell line adherent plasmid stable transfection Metafectene PRO publication V. Seiberlich et al., Cell Biochem. Biophys., 2013, 67: 149–160, DOI 10.1007/s12013-013-9622-8

OLN93

Rat oligodendroglial cells Rat nervous system unknown adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication C. Brill et al., Scientific Reports, 7:43000, DOI: 10.1038/srep43000

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