References
Products Transfection
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Instruction for Use:
In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.
No reference? > General Selection Guide
If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.
| CELL TYPE | DESCRIPTION OF CELL TYPE | CAT.NO. | SPECIES | ORIGIN OF CELLS | COMMON CELL TYPE | GROWTH PROPERTIES | NUCLEIC ACID / DELIVERED MOLECULE | APPLICATION | PRODUCT | REFERENCE TYPE | BIBLIOGRAPHIC DATA | LINK | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mouse DRG neurons |
Mouse peripheral dorsal root ganglia neurons | Mouse | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | X. Jing et al., Exp. Neurol., 2012, 233 (1): 221–232, doi:10.1016/j.expneurol.2011.10.005 | Link | ||
L1–L6 DRG |
Mouse lumbar dorsal root ganglia (DRG) | Mouse | nervous system | primary cell | adherent | plasmid | cotransfection (plasmid/plasmid) | Metafectene PRO | publication | C. Bavassano et al., Biochim. et Biophysica Acta, 2013, 1833: 3166–3175, doi.org/10.1016/j.bbamcr.2013.09.001 | Link | ||
HOG |
Human oligodendroglial cells | Human | nervous system | unknown | adherent | plasmid | Metafectene | publication | G. Chase et al., J. Virol., Jan 2007; 81: 743 - 749 | Link | |||
D283 Med |
Human medulloblastoma cell line | ATCC HTB-185 | Human | nervous system | cell line | suspension | plasmid | Metafectene | publication | S. Couillard-Despres et al., 2014, US 8,841,430 | Link | ||
aNS |
Adult Neurospheres (Sphere-forming cells isolated from the lateral walls of the lateral ventricle of C57BL/6 mice) | Mouse | nervous system | unknown | siRNA | RNA-Interference | Metafectene PRO | publication | B. M. Grebbin et al., Development, Jul 2016; 143: 2281 - 2291 | Link | |||
aNS |
Adult Neurospheres (Spere-forming cells isolated from lateral walls of lateral ventricle of C57BL/6 mice) | Mouse | nervous system | unknown | siRNA | 3D cell culture | Metafectene PRO | publication | B. M. Grebbin et al., Development, Jul 2016; 143: 2281 - 2291 | Link | |||
46C-derived NSCs |
Mouse 46C-derived radial glia like neural stem cells | Mouse | nervous system | stem cell | adherent | plasmid | Metafectene EASY | publication | S. Pachernegg et al., PLOSONE, 2018, doi.org/10.1371/journal.pone.0192242 | Link | |||
| Primary rat neuronal dendrites | Rat | nervous system | primary cell | adherent | mRNA | Metafectene | publication | J. Eberwine et al., 2013, US 8,367.402 | |||||
| Rat (Sprague Dawley young adult male) primary sensory neurons | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335 | Link | |||
| Rat (Sprague Dawley young adult male) primary sensory neurons | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529 | Link | |||
| Rat sensory neurons isolated from dorsal root ganglia (DRG) | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 | Link | |||
| Rat (Sprague Dawley young adult male) primary sensory neurons | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748 | Link |
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