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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent plasmid Metafectene PRO publication S.-H. Hong et al., Oncotarget, 2016, 7 (40): 65335-65347 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent plasmid Metafectene PRO publication C. Maucksch et al., Nucleic Acids Res., Oct 2008; 36: 5462 - 5471 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent plasmid Metafectene PRO publication F. L. Pennemann et al., Nature Commun., 2021, 12: 7009, doi.org/10.1038/s41467-021-27192-w Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication Z. Zeng et al., DNA Repair, October 2017, 58: 52-61

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent plasmid Metafectene PRO publication H. N. Pfäffle et al., Cancer Res., Oct 2013; 73: 6254 - 6263 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication C. Kerzendorfer et al., Hum. Mol. Genet., Apr 2010; 19: 1324 - 1334 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication P. Hubel et al., Nature Immunology, 2019, 20: 493–502, https://doi.org/10.1038/s41590-019-0323-3. Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent dsRNA RNA-Interference Metafectene PRO publication F. L. Pennemann et al., Nature Commun., 2021, 12: 7009, doi.org/10.1038/s41467-021-27192-w Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication S. L. Rulten et al., Nucleic Acids Res., Jan 2014; 42: 307 - 314 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication A. Tsolou et al., Cancer Biol. Med., 2017, 14 (3): 293-301, doi: 10.20892/j.issn.2095-3941.2017.0049 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication I. V. Karagounis et al., British Journal of Cancer, 2016, 115: 312-321; doi: 10.1038/bjc.2016.202 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication H. Brunton et al., Mol. Cell. Biol., Oct 2011; 31: 4022 - 4035 Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent plasmid cotransfection (plasmid/plasmid) Metafectene PRO publication M. Hülskötter, Dissertation, 2012, University of Hamburg Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent oligonucleotide Metafectene PRO publication F. L. Pennemann et al., Nature Commun., 2021, 12: 7009, doi.org/10.1038/s41467-021-27192-w Link

A549

Human lung alveolar epithelial adenocarcinoma carcinoma cell line ATCC CCL-185 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication M. .I Koukourakis et al., Laboratory Investigation, 2017, 97: 1321-1331, doi:10.1038/labinvest.2017.79

BEAS-2B

Human bronchial epithelial cell line ATCC CRL-9609 Human respiratory system cell line adherent plasmid Metafectene PRO publication C. Maucksch et al., Nucleic Acids Res., Oct 2008; 36: 5462 - 5471 Link

HCC-44

Human lung adenocarcinoma cell line Human respiratory system cell line adherent plasmid Metafectene PRO publication J. Berrout et al., Nature Communications, 2017,8: 947, DOI: 10.1038/s41467-017-00983-w Link

HCC-515

Human lung adenocarcinoma cell line Human respiratory system cell line adherent plasmid Metafectene PRO publication J. Berrout et al., Nature Communications, 2017,8: 947, DOI: 10.1038/s41467-017-00983-w Link

HLF

Human lung fibroblasts Human respiratory system primary cell adherent siRNA RNA-Interference Metafectene PRO publication X. Deng et al., J. Cell. Biochem., 2017, 118: 4012-4019, DOI 10.1002/jcb.26057

JEG-3

Human placenta choriocarcinoma cell line ATCC HTB-36 Human respiratory system cell line adherent plasmid Metafectene PRO publication J. E. D. Escobar, Universität Hamburg Link

LLC1

Lewis Lung carcinoma cell line (Mouse) Mouse respiratory system cell line adherent plasmid Metafectene PRO publication H.F. Alkan et al., bioRxiv preprint, 2020, doi.org/10.1101/2020.05.28.114629 Link

MRC5

Human fetal lung fibroblast cell line ATCC CCL-171 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication S.-C. Chiang, University of Sussex Link

MRC5

Human fetal lung fibroblast cell line ATCC CCL-171 Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication M. .I Koukourakis et al., Laboratory Investigation, 2017, 97: 1321-1331, doi:10.1038/labinvest.2017.79

MRC5VA

Human SV40-transformed lung fibroblast cell line Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication I. Abramowicz ez al., HMG Advance Access, 2016

MRC5VA

Human SV40-transformed lung fibroblast cell line Human respiratory system cell line adherent siRNA RNA-Interference Metafectene PRO publication I. Abramowicz et al., Hum. Mol. Genet., Dec 2016, DOI: 10.1093/hmg/ddw364

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