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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent oligonucleotide Metafectene publication H. Mietz et al., Invest. Ophthalmol. Vis. Sci., May 2003; 44: 4290

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent plasmid stable transfection Metafectene PRO publication C. Morich, Dissertation, 2016, Georg-August University Göttingen Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent bacmid Metafectene PRO publication N. Fourier et al., bioRxiv, 2018, doi.org/10.1101/396291 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent plasmid Metafectene PRO publication R. Costa., Front. Cell Dev. Biol., 2020, 8: 607080, doi: 10.3389/fcell.2020.607080 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent plasmid Metafectene publication A. Kröger et al., Cancer Res., Apr 2007; 67: 2972 - 2981 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent plasmid Metafectene PRO publication R. Costa et al., Cell Physiol. Biochem., 2018, 45: 867-882 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent plasmid Metafectene publication A. Stirnweiss et al., J. Immunol., May 2010; 184: 5179 - 5185 Link

Neuroscreen-1

Rat adrenal gland pheochromocytoma cell line (derivate of PC12) Rat unknown cell line adherent plasmid Metafectene publication N. .J. Bradshaw et al., PLoS ONE, 2014, 9 (10): e111196, doi:10.1371/journal.pone.0111196 Link

NCI-H290

Human pleural malignant mesothelioma cell line Human unknown cell line plasmid stable transfection Metafectene publication S. Abe et al., Cancer Sci., 2016, 107: 1198-1205; doi: 10.1111/cas.12985 Link

MRC5VI

Human fibroblast cell line, SV40 transformed and immortalised Human unknown cell line adherent plasmid Metafectene PRO publication S. Sertic et al., PNAS, Aug 2011; 108: 13647 - 13652 Link

Mouse VDEC

Mouse vas deferens epithelial cells Mouse unknown unknown adherent siRNA RNA-Interference Metafectene publication L. Léotoing et al., J. Mol. Endocrinol., Aug 2007; 39: 151 - 162 Link

mESC

Mouse embryonic stem cells Mouse unknown stem cell adherent plasmid RNA-Interference Metafectene PRO publication SY. Kim, Thesis, 2015, Seoul National University Link

mESC

Mouse embryonic stem cells Mouse unknown stem cell adherent plasmid Metafectene PRO publication X. Duportet et al., Nucleic Acids Res., Dec 2014; 42: 13440 - 13451 Link

mESC

Mouse embryonic stem cells Mouse unknown stem cell adherent plasmid Metafectene PRO publication T. Schmidt, Dissertation, 2012, Julius-Maximilians-Universität Würzburg Link

mESC

Mouse embryonic stem cells Mouse unknown stem cell adherent plasmid stable transfection Metafectene PRO publication M. Hein, Doctoral thesis, 2014, Julius-Maximilians-Universitaet Wuerzburg Link

mESC

Mouse embryonic stem cells Mouse unknown stem cell adherent plasmid Metafectene PRO publication X. Duportet, Dissertation, 2014, Université Paris Diderot Link

mESC

Mouse embryonic stem cells Mouse unknown stem cell plasmid Metafectene PRO publication R. Weiss et al., 2019, US 2019 / 0032141 A1 Link

MEF Rb -/-

Mouse embryonic fibroblasts, deficent of Rb Mouse unknown unknown adherent plasmid Metafectene publication P. Hallenborg et al., Mol. Cell. Biol., Aug 2010; 30: 4077 - 4091 Link

MEF PTP-PEST -/-

Mouse embryonic fibroblasts, deficient of PTP-Pest Mouse unknown unknown adherent plasmid Metafectene publication J. S. Jamieson et al., J. Cell Sci., Dec 2005; 118: 5835 - 5847 Link

MEF paxillin–/–

Mouse embryonic fibroblasts, deficient of paxillin Mouse unknown unknown adherent plasmid Metafectene publication J. S. Jamieson et al., J. Cell Sci., Dec 2005; 118: 5835 - 5847 Link

MEF p62 -/-

Mouse embryonic fibroblasts, deficient of p62 (derivat of MEF) Mouse unknown unknown adherent plasmid Metafectene PRO publication A. Jain et al., J. Biol. Chem., Jul 2010; 285: 22576 - 22591 Link

MEF p38alpha -/-

Mouse embryonic fibroblasts, deficient of p38alpha (derivat of MEF) Mouse unknown unknown adherent plasmid Metafectene PRO publication Á.Gutiérrez-Uzquiza et al., J. Biol. Chem., Jan 2012; 287: 2632 - 2642 Link

MEF p38alpha -/-

Mouse embryonic fibroblasts, deficient of p38alpha (derivat of MEF) Mouse unknown unknown adherent plasmid Metafectene PRO publication M. Arechederra et al., J. Biol. Chem., Feb 2015; 290: 4383 - 4397

MEF LMNA -/-

Mouse embryonic fibroblasts (deficient of LMNA) Mouse unknown unknown adherent plasmid Metafectene PRO publication E. Bartova et al., Protoplasma, 2017, 254: 2035-2043, DOI 10.1007/s00709-017-1076-1

MEF IFNAR -/-

Mouse embryonic fibroblasts, deficient of IFNAR Mouse unknown unknown adherent dsRNA Metafectene publication M. Habjan et al., J. Virol., May 2009; 83: 4365 - 4375. Link

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