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Species
  1. Cow (3)
  2. Human (20)
  3. Mouse (3)
  4. Rat (2)
  5. Swine (4)
  6. unknown (1)
Common cell type
  1. cell line (5)
  2. primary cell (22)
  3. unknown (6)
Nucleic acid / Delivered molecule
  1. plasmid (20)
  2. oligonucleotide (2)
  3. siRNA (11)
Application
  1. stable transfection (1)
  2. RNA-Interference (12)
  3. cotransfection (plasmid/plasmid) (3)
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  1. Metafectene (12)
  2. Metafectene PRO (18)
  3. Metafectene SI (1)
  4. DOTAP (2)

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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Rat VSMC

 Rat (Wistar) vascular smooth muscle cells from thoracic aorta Rat vasculature unknown siRNA RNA-Interference Metafectene publication A. Giordano et al., Am J Physiol Heart Circ Physiol, Jun 2006; 290: H2459 - H2465 Link

Rat VSMC

 Rat vascular smooth muscle cells Rat vasculature unknown plasmid DOTAP publication W. Li et al., Am J Physiol Heart Circ Physiol, Jan 2005; 288: H408 - H415

pPSMC

 Porcine primary smooth muscle cells Swine vasculature primary cell adherent plasmid Metafectene publication J. Pelisek et al., J Mol Med (Berl), Nov 2002; 80(11): 724-36

pPPAEC

 Porcine primary pulmonary artery endothelial cells Swine vasculature primary cell adherent plasmid Metafectene publication B. Wojciak-Stothard et al., Am J Physiol Lung Cell Mol Physiol, Apr 2005; 288: L749 - L760 Link

pBAEC

 Bovine primary aortic endothelial cells Cow vasculature primary cell adherent plasmid Metafectene publication K. Renner at al., J. Cell. Physiol., 2012, 227: 398–2411, DOI: 10.1002/jcp.22975

pBAEC

 Bovine primary aortic endothelial cells Cow vasculature primary cell adherent plasmid Metafectene PRO publication A. Nayebosadri et al., Am J Physiol Cell Physiol, Aug 2013; 305: C309 - C322 Link

pBAEC

 Bovine primary aortic endothelial cells Cow vasculature primary cell adherent siRNA RNA-Interference Metafectene publication Y. Riahi et al., Diabetes, Apr 2010; 59: 808 - 818 Link

PASMC

 Pulmonary artery smooth muscle cells unknown vasculature unknown plasmid Metafectene publication W. Zhang et al., Sheng Li Xue Bao, Feb 2006; 58(1): 71-6

PAEC

 Porcine primary aortic endothelial cells Swine vasculature primary cell adherent plasmid Metafectene publication B. Wojciak-Stothard et al., Am J Physiol Lung Cell Mol Physiol, Jun 2006; 290: L1173 - L1182 Link

PAEC

 Porcine primary aortic endothelial cells Swine vasculature primary cell adherent plasmid Metafectene PRO publication B. Wojciak-Stothard et al., J.Cell Sci, March 2007;120: 929-942 Link

MOVAS-1

 Mouse aorta smooth muscle cells ATCC CRL-2797 Mouse vasculature cell line adherent plasmid cotransfection (plasmid/plasmid) Metafectene PRO publication B. Park et al., Bioscience, Biotechnology, and Biochemistry, 2015, 79(4): 539-552; DOI: 10.1080/09168451.2014.991681 Link

MOVAS-1

 Mouse aorta smooth muscle cells ATCC CRL-2797 Mouse vasculature cell line adherent plasmid cotransfection (plasmid/plasmid) Metafectene PRO publication K. Lee et al., Arch. Pharm. Res., 2016, 39:83-93; DOI 10.1007/s12272-015-0687-3

MOVAS-1

 Mouse aorta smooth muscle cells ATCC CRL-2797 Mouse vasculature cell line adherent siRNA RNA-Interference Metafectene PRO publication B. Park et al., Bioscience, Biotechnology, and Biochemistry, 2015, 79(4): 539-552; DOI: 10.1080/09168451.2014.991681 Link

hVSMC

 Human primary vascular smooth muscle cells Human vasculature primary cell adherent plasmid stable transfection Metafectene publication G. Weisinger et al., Exp. Cell Res., 2013, 319: 1586-1593, doi.org/10.1016/j.yexcr.2013.04.001

hVSMC

 Human vascular (aortic) smooth muscle cells Human vasculature unknown adherent siRNA RNA-Interference Metafectene publication A. Giordano et al., Cardiovasc Res, Aug 2008; 79: 519 - 526 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent siRNA RNA-Interference Metafectene PRO publication H.-Y. Lee et al., Korean Circ. J., 2012, 42: 239-245, doi.org/10.4070/kcj.2012.42.4.239 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent plasmid Metafectene PRO publication N. Kostopoulou et al., J. Cell Sci., 2021, 134, jcs246892, doi:10.1242/jcs.246892 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent siRNA RNA-Interference Metafectene PRO publication H.-Y. Lee et al., Cardiovasc Res, Jan 2013; 97: 143 - 152

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent plasmid Metafectene PRO publication J. Yang et al., Oncology Reports, 2015, 34: 2745-2751; DOI: 10.3892/or.2015.4223 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent siRNA RNA-Interference Metafectene publication A. Giordano et al., Am J Physiol Heart Circ Physiol, Jan 2012; 302: H135 - H142 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication S. Thakker et al., Oncotarget, 2018, 9 (1): 1210-1228 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent siRNA RNA-Interference Metafectene PRO publication J. Park et al., Biochem. and Biophys. Res. Commun., 2018, 495: 2050-2057, doi.org/10.1016/j.bbrc.2017.08.023

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent plasmid Metafectene PRO publication S. Bellou et al., Am J Physiol Cell Physiol, Dec 2009; 297: C1477 - C1489

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent plasmid Metafectene PRO publication S.-W. Lee et al., Molecular Therapy, 2013, 21 (9): 1767–1777, doi:10.1038/mt.2013.146 Link

HUVEC

 Human primary umbilical vein endothelial cells ATCC CRL-1730 Human vasculature primary cell adherent oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

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