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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

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If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Rat primary hepatocytes Rat digestive organs primary cell adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

Rat primary hepatocytes Rat digestive organs primary cell adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication R. Domínguez-Kelly et al., J. Cell Biol., Aug 2011; 194: 567 - 579 Link

ATC1

Murine adrenocortical tumor cell line Murine other cell line plasmid cotransfection (plasmid/siRNA) Metafectene publication A.-M. Lefrancois-Martinez et al., J. Biol. Chem., 2011, 286 (38) : 32976–32985, DOI 10.1074/jbc.M111.218017 Link

CCD 841 CoTr

Human normal colon epithel cell line ATCC CRL-1807 Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

CCD 841 CoTr

Human normal colon epithel cell line ATCC CRL-1807 Human digestive organs cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

COS-1

African green monkey kidney fibroblast-like cell line ATCC CRL-1650 Monkey urinary system cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication J.-S. Chung et al., J. Immunol., Nov 2007; 179: 5778 - 5784 Link

COS-1

African green monkey kidney fibroblast-like cell line ATCC CRL-1650 Monkey urinary system cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication J.-S. Chung et al., J. Immunol., Nov 2007; 179: 5778 - 5784 Link

COS-7

African green monkey kidney fibroblast-like cell line ATCC CRL-1651 Monkey urinary system cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication A. Rieger at al., Bioforum 12, 846, 2002

COS-7

African green monkey kidney fibroblast-like cell line ATCC CRL-1651 Monkey urinary system cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication A. Rieger at al., Bioforum 12, 846, 2002

D54

Human brain glioblastoma cell line Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

D54

Human brain glioblastoma cell line Human brain cell line adherent plasmid cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

HBL 100

Human transformed epithelial cell line Human breast cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication A. M. Scherbakov et al., Exp. Cell Res., 2013, 319: 3150 – 3159, doi.org/10.1016/j.yexcr.2013.08.019

HBL 100

Human transformed epithelial cell line Human breast cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication A. M. Scherbakov et al., Exp. Cell Res., 2013, 319: 3150 – 3159, doi.org/10.1016/j.yexcr.2013.08.019

HCT116

Human colon adenocarcinoma cell line ATCC CCL-247 Human digestive organs cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

HCT116

Human colon adenocarcinoma cell line ATCC CCL-247 Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

HEK293

Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication M.-J. Stahnke et al., Cellular Signalling, 2014, 26 :1792-1799doi.org/10.1016/j.cellsig.2014.04.006

HeLa

Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication X. M. M. Weyel, Dissertation, 2018, Goethe- Universität in Frankfurt am Main Link

HeLa

Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication X. M. M. Weyel, Dissertation, 2018, Goethe- Universität in Frankfurt am Main Link

HepG2

Human hepatocellular carcinoma cell line ATCC HB-8065 Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

HepG2

Human hepatocellular carcinoma cell line ATCC HB-8065 Human digestive organs cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

HIT-T15

Hamster pancreas beta cell line ATCC CRL-1777 Hamster digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication S. Boerchers, Dissertation, 2014, Georg-August-Universitaet zu Goettingen

HIT-T15

Hamster pancreas beta cell line ATCC CRL-1777 Hamster digestive organs cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication S. Börchers et al., Naunyn-Schmiedeberg's Arch. Pharmacol., 2017, 390: 813-825, DOI 10.1007/s00210-017-1385-0

HIT-T15

Hamster pancreas beta cell line ATCC CRL-1777 Hamster digestive organs cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication S. Boerchers, Dissertation, 2014, Georg-August-Universitaet zu Goettingen

HIT-T15

Hamster pancreas beta cell line ATCC CRL-1777 Hamster digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication S. Börchers et al., Naunyn-Schmiedeberg's Arch. Pharmacol., 2017, 390: 813-825, DOI 10.1007/s00210-017-1385-0

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