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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

WI-38

Human lung fibroblast cells ATCC CCL-75 Human respiratory system unknown adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 Link

U87 MG

Human brain glioblastoma cell line ATCC HTB-14 Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication M. Nabissi et al., Carcinogenesis, May 2010; 31: 794 - 803 Link

U87 MG

Human brain glioblastoma cell line ATCC HTB-14 Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene EASY publication M. Nabissi et al., Carcinogenesis, Jan 2013; 34: 48 - 57 Link

U2OS

Human bone osteosarcoma cell line ATCC HTB-96 Human bone cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication S. Hernández-Pérez et al., Oncogene, 2017, 36: 4802-4809, doi:10.1038/onc.2017.21

U2OS

Human bone osteosarcoma cell line ATCC HTB-96 Human bone cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication Y. Martin et al., Oncogene, 2015, 34:1058-1063; doi:10.1038/onc.2014.38

U251

Human brain glioblastoma cell line Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

TM-GBM

Human Glioma spheroides obtained by microdissection of U87MG xenografted mice brain Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

SH-SY5Y

Human neuroblastoma cell line ATCC CRL-2266 Human bone cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication S. Kim et al., Mol. Cell Biol., 2015, 35:1754-1762; doi:10.1128/MCB.01325-14 Link

OLN93

Rat oligodendroglial cells Rat nervous system unknown adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication C. Brill et al., Scientific Reports, 7:43000, DOI: 10.1038/srep43000

NSC34

Mouse motor neuron-like hybrid cell line Mouse unknown cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication N. Hensel et al., Cellular Signalling, 2014, 26: 540–548; doi.org/10.1016/j.cellsig.2013.11.027

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication P. K. Kwon et al., The FASEB Journal.,2021, 35: e21507, DOI: 10.1096/fj.202002076R Link

MZC

Human primary glioblastoma cells Human brain primary cell siRNA cotransfection (plasmid/siRNA) Metafectene EASY publication M. Nabissi et al., Carcinogenesis, Jan 2013; 34: 48 - 57 Link

MZC

Human primary glioblastoma cells Human brain primary cell siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication M. Nabissi et al., Carcinogenesis, May 2010; 31: 794 - 803 Link

MDCK

Madin darby canine kidney cell line ATCC CCL 34 Dog urinary system cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication S. Liu et al., J. Biol. Chem., 2015, 290(29): 17894-17908; DOI 10.1074/jbc.M114.607184 Link

MDA-MB-231

Human breast adenocarcinoma cell line ATCC HTB-26 Human breast cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication S. Liu et al., J. Biol. Chem., 2015, 290(29): 17894-17908; DOI 10.1074/jbc.M114.607184 Link

LoVo

Human colon adenocarcinoma cell line ATCC CCL-229 Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

IMR-90

Human primary lung fibroblasts ATCC CCL-186 Human respiratory system primary cell adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 Link

hTSMC

Human primary tracheal smooth muscle cells Human respiratory system primary cell adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication W.-N. Lin et al., Brit. J. Pharmacol., 2011, 163: 1691–1706, DOI:10.1111/j.1476-5381.2011.01312.x Link

HT-29

Human colorectal adenocarcinoma cell line ATCC HTB-38 Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

HKe-3

Human colon carcinoma cell line Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

HIT-T15

Hamster pancreas beta cell line ATCC CRL-1777 Hamster digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication S. Boerchers, Dissertation, 2014, Georg-August-Universitaet zu Goettingen

HIT-T15

Hamster pancreas beta cell line ATCC CRL-1777 Hamster digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication S. Börchers et al., Naunyn-Schmiedeberg's Arch. Pharmacol., 2017, 390: 813-825, DOI 10.1007/s00210-017-1385-0

HepG2

Human hepatocellular carcinoma cell line ATCC HB-8065 Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

HeLa

Human cervix adenocarcinoma cell line ATCC CCL-2 Human genital tract cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication X. M. M. Weyel, Dissertation, 2018, Goethe- Universität in Frankfurt am Main Link

HCT116

Human colon adenocarcinoma cell line ATCC CCL-247 Human digestive organs cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland Link

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