References
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Instruction for Use:
In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.
No reference? > General Selection Guide
If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.
CELL TYPE | DESCRIPTION OF CELL TYPE | CAT.NO. | SPECIES | ORIGIN OF CELLS | COMMON CELL TYPE | GROWTH PROPERTIES | NUCLEIC ACID / DELIVERED MOLECULE | APPLICATION | PRODUCT | REFERENCE TYPE | BIBLIOGRAPHIC DATA | LINK | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
WI-38 |
Human lung fibroblast cells | ATCC CCL-75 | Human | respiratory system | unknown | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 | Link | |
U87 MG |
Human brain glioblastoma cell line | ATCC HTB-14 | Human | brain | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | M. Nabissi et al., Carcinogenesis, May 2010; 31: 794 - 803 | Link | |
U87 MG |
Human brain glioblastoma cell line | ATCC HTB-14 | Human | brain | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene EASY | publication | M. Nabissi et al., Carcinogenesis, Jan 2013; 34: 48 - 57 | Link | |
U2OS |
Human bone osteosarcoma cell line | ATCC HTB-96 | Human | bone | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | S. Hernández-Pérez et al., Oncogene, 2017, 36: 4802-4809, doi:10.1038/onc.2017.21 | ||
U2OS |
Human bone osteosarcoma cell line | ATCC HTB-96 | Human | bone | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | Y. Martin et al., Oncogene, 2015, 34:1058-1063; doi:10.1038/onc.2014.38 | ||
U251 |
Human brain glioblastoma cell line | Human | brain | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | K2 Transfection System | publication | P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 | Link | ||
TM-GBM |
Human Glioma spheroides obtained by microdissection of U87MG xenografted mice brain | Human | brain | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | K2 Transfection System | publication | P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 | Link | ||
SH-SY5Y |
Human neuroblastoma cell line | ATCC CRL-2266 | Human | bone | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | S. Kim et al., Mol. Cell Biol., 2015, 35:1754-1762; doi:10.1128/MCB.01325-14 | Link | |
OLN93 |
Rat oligodendroglial cells | Rat | nervous system | unknown | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | C. Brill et al., Scientific Reports, 7:43000, DOI: 10.1038/srep43000 | |||
NSC34 |
Mouse motor neuron-like hybrid cell line | Mouse | unknown | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | N. Hensel et al., Cellular Signalling, 2014, 26: 540–548; doi.org/10.1016/j.cellsig.2013.11.027 | |||
NIH/3T3 |
Mouse embryonic fibroblast cell line | ATCC CRL-1658 | Mouse | unknown | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | P. K. Kwon et al., The FASEB Journal.,2021, 35: e21507, DOI: 10.1096/fj.202002076R | Link | |
MZC |
Human primary glioblastoma cells | Human | brain | primary cell | siRNA | cotransfection (plasmid/siRNA) | Metafectene EASY | publication | M. Nabissi et al., Carcinogenesis, Jan 2013; 34: 48 - 57 | Link | |||
MZC |
Human primary glioblastoma cells | Human | brain | primary cell | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | M. Nabissi et al., Carcinogenesis, May 2010; 31: 794 - 803 | Link | |||
MDCK |
Madin darby canine kidney cell line | ATCC CCL 34 | Dog | urinary system | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | S. Liu et al., J. Biol. Chem., 2015, 290(29): 17894-17908; DOI 10.1074/jbc.M114.607184 | Link | |
MDA-MB-231 |
Human breast adenocarcinoma cell line | ATCC HTB-26 | Human | breast | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | S. Liu et al., J. Biol. Chem., 2015, 290(29): 17894-17908; DOI 10.1074/jbc.M114.607184 | Link | |
LoVo |
Human colon adenocarcinoma cell line | ATCC CCL-229 | Human | digestive organs | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland | Link | |
IMR-90 |
Human primary lung fibroblasts | ATCC CCL-186 | Human | respiratory system | primary cell | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 | Link | |
hTSMC |
Human primary tracheal smooth muscle cells | Human | respiratory system | primary cell | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | W.-N. Lin et al., Brit. J. Pharmacol., 2011, 163: 1691–1706, DOI:10.1111/j.1476-5381.2011.01312.x | Link | ||
HT-29 |
Human colorectal adenocarcinoma cell line | ATCC HTB-38 | Human | digestive organs | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland | Link | |
HKe-3 |
Human colon carcinoma cell line | Human | digestive organs | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland | Link | ||
HIT-T15 |
Hamster pancreas beta cell line | ATCC CRL-1777 | Hamster | digestive organs | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | S. Boerchers, Dissertation, 2014, Georg-August-Universitaet zu Goettingen | ||
HIT-T15 |
Hamster pancreas beta cell line | ATCC CRL-1777 | Hamster | digestive organs | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | S. Börchers et al., Naunyn-Schmiedeberg's Arch. Pharmacol., 2017, 390: 813-825, DOI 10.1007/s00210-017-1385-0 | ||
HepG2 |
Human hepatocellular carcinoma cell line | ATCC HB-8065 | Human | digestive organs | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906 | ||
HeLa |
Human cervix adenocarcinoma cell line | ATCC CCL-2 | Human | genital tract | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | X. M. M. Weyel, Dissertation, 2018, Goethe- Universität in Frankfurt am Main | Link | |
HCT116 |
Human colon adenocarcinoma cell line | ATCC CCL-247 | Human | digestive organs | cell line | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene | publication | L. M. Williams, Dissertation, 2014, Royal College of Surgeons in Ireland | Link |