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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

WI-38

Human lung fibroblast cells ATCC CCL-75 Human respiratory system unknown adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 Link

WI-38

Human lung fibroblast cells ATCC CCL-75 Human respiratory system unknown adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication A. M. Szekely et al., Mol. Cell. Biol., Dec 2005; 25: 10492 - 10506 Link

U87 MG

Human brain glioblastoma cell line ATCC HTB-14 Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene EASY publication M. Nabissi et al., Carcinogenesis, Jan 2013; 34: 48 - 57 Link

U87 MG

Human brain glioblastoma cell line ATCC HTB-14 Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication M. Nabissi et al., Carcinogenesis, May 2010; 31: 794 - 803 Link

U87 MG

Human brain glioblastoma cell line ATCC HTB-14 Human brain cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication S. Liu et al., J. Biol. Chem., 2015, 290(29): 17894-17908; DOI 10.1074/jbc.M114.607184 Link

U87 MG

Human brain glioblastoma cell line ATCC HTB-14 Human brain cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene EASY publication M. Nabissi et al., Carcinogenesis, Jan 2013; 34: 48 - 57 Link

U87 MG

Human brain glioblastoma cell line ATCC HTB-14 Human brain cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication M. Nabissi et al., Carcinogenesis, May 2010; 31: 794 - 803 Link

U2OS

Human bone osteosarcoma cell line ATCC HTB-96 Human bone cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication Y. Martin et al., Oncogene, 2015, 34:1058-1063; doi:10.1038/onc.2014.38

U2OS

Human bone osteosarcoma cell line ATCC HTB-96 Human bone cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication S. Hernández-Pérez et al., Oncogene, 2017, 36: 4802 4809, doi:10.1038/onc.2017.21

U2OS

Human bone osteosarcoma cell line ATCC HTB-96 Human bone cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication Y. Martin et al., Oncogene, 2015, 34:1058-1063; doi:10.1038/onc.2014.38

U2OS

Human bone osteosarcoma cell line ATCC HTB-96 Human bone cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication S. Hernández-Pérez et al., Oncogene, 2017, 36: 4802-4809, doi:10.1038/onc.2017.21

U251

Human brain glioblastoma cell line Human brain cell line adherent plasmid cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

U251

Human brain glioblastoma cell line Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

TM-GBM

Human Glioma spheroides obtained by microdissection of U87MG xenografted mice brain Human brain cell line adherent plasmid cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

TM-GBM

Human Glioma spheroides obtained by microdissection of U87MG xenografted mice brain Human brain cell line adherent siRNA cotransfection (plasmid/siRNA) K2 Transfection System publication P. D’Arrigo et al., Cell Death Discovery, 2019, 5:137, doi.org/10.1038/s41420-019-0216-0 Link

SH-SY5Y

Human neuroblastoma cell line ATCC CRL-2266 Human bone cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication S. Kim et al., Mol. Cell Biol., 2015, 35:1754-1762; doi:10.1128/MCB.01325-14 Link

SH-SY5Y

Human neuroblastoma cell line ATCC CRL-2266 Human bone cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication S. Kim et al., Mol. Cell Biol., 2015, 35:1754-1762; doi:10.1128/MCB.01325-14 Link

OLN93

Rat oligodendroglial cells Rat nervous system unknown adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication C. Brill et al., Scientific Reports, 7:43000, DOI: 10.1038/srep43000

OLN93

Rat oligodendroglial cells Rat nervous system unknown adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication C. V. Brill, Dissertation, 2018, Charité – Universitätsmedizin Berlin Link

OLN93

Rat oligodendroglial cells Rat nervous system unknown adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication C. V. Brill, Dissertation, 2018, Charité – Universitätsmedizin Berlin Link

OLN93

Rat oligodendroglial cells Rat nervous system unknown adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication C. Brill et al., Scientific Reports, 7:43000, DOI: 10.1038/srep43000

NSC34

Mouse motor neuron-like hybrid cell line Mouse unknown cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication N. Hensel et al., Cellular Signalling, 2014, 26: 540–548; doi.org/10.1016/j.cellsig.2013.11.027

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication S. Liu et al., J. Biol. Chem., 2015, 290(29): 17894-17908; DOI 10.1074/jbc.M114.607184 Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent plasmid cotransfection (plasmid/siRNA) Metafectene publication P. K. Kwon et al., The FASEB Journal.,2021, 35: e21507, DOI: 10.1096/fj.202002076R Link

NIH/3T3

Mouse embryonic fibroblast cell line ATCC CRL-1658 Mouse unknown cell line adherent siRNA cotransfection (plasmid/siRNA) Metafectene publication P. K. Kwon et al., The FASEB Journal.,2021, 35: e21507, DOI: 10.1096/fj.202002076R Link

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