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Reference type
  1. publication (42)
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  1. Human (37)
  2. Mouse (3)
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  1. bone (2)
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  3. digestive organs (11)
  4. genital tract (8)
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  8. urinary system (12)
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  1. cell line (41)
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  1. plasmid (32)
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  1. Metafectene (7)
  2. Metafectene PRO (23)
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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

293A

 Human embryonic kidney adenovirus producer cell line (derivat of HEK293) DSM ACC 305 Human urinary system cell line adherent sgRNA CRISPR Metafectene SI publication K. Kang et al., J. of Animal Sci. and Biotech., 2019, doi.org/10.1186/s40104-019-0354-510: 43 Link

293T

 Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid CRISPR K4 Transfection System publication D. Marks et al., bioRxiv preprint, 2021, doi.org/10.1101/2021.07.01.450700 Link

293T

 Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent sgRNA CRISPR K4 Transfection System publication D. Marks et al., bioRxiv preprint, 2021, doi.org/10.1101/2021.07.01.450700 Link

293T

 Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent plasmid CRISPR K2 Transfection System publication D. Marks et al., bioRxiv preprint, 2021, doi.org/10.1101/2021.09.15.460502 Link

6E4 HeLaT

 Human cervix adenocarcinoma cell line (derivative of HeLa) Human genital tract cell line adherent sgRNA CRISPR Metafectene publication D. Kim et al., Nat ure Struct ural & Molec ular Biology, 2020, 27: 581–588, doi.org/10.1038/s41594-020-0427-3

6E4 HeLaT

 Human cervix adenocarcinoma cell line (derivative of HeLa) Human genital tract cell line adherent plasmid CRISPR Metafectene publication D. Kim et al., Nat ure Struct ural & Molec ular Biology, 2020, 27: 581–588, doi.org/10.1038/s41594-020-0427-3

A375

 Human skin malignant melanoma cell line ATCC CRL-1619 Human skin cell line adherent plasmid CRISPR K2 Transfection System publication M. Tufano et al., Front. Cell Dev. Biol., 2021, 9: 718947, doi: 10.3389/fcell.2021.718947 Link

B16F10

 Mouse melanoma cell line ATCC CRL-6475 Mouse skin cell line adherent plasmid CRISPR K2 Transfection System publication K. Yokomizo et al., Med. Oncology, 2022, 39: 58, doi.org/10.1007/s12032-022-01659-2 Link

Caco-2

 Human colon adenocarcinoma cell line ATCC HTB-37 Human digestive organs cell line adherent plasmid CRISPR Metafectene publication Y.-R. Kim et al., J. Cell. Mol. Med., 2017, 21 (12): 3565-3578, doi: 10.1111/jcmm.13267 Link

CT26

 Mouse colon carcinoma cell line ATCC CRL-2638 Mouse digestive organs cell line adherent plasmid CRISPR K2 Transfection System publication K. Yokomizo et al., Med. Oncology, 2022, 39: 58, doi.org/10.1007/s12032-022-01659-2 Link

DLD-1

 Human colon adenocarcinoma cell line ATCC CCL-221 Human digestive organs cell line adherent plasmid CRISPR Metafectene publication C. Emming, Dissertation, 2021, Ludwig-Maximilians-Universität München Link

Flp-In T-REx HeLa

 Human cervix adenocarcinoma cell line (derivative of HeLa) Human genital tract cell line adherent plasmid CRISPR Metafectene PRO publication T. M. Nthiga et al., J. Cell Biol., 2021, 220, 6, doi.org/10.1083/jcb.202006128 Link

Flp-in TREx 293

 Human embryonic kidney cell line (derivative of HEK293) Human urinary system cell line adherent plasmid CRISPR Metafectene PRO publication T. M. Nthiga et al., The EMBO J., 39: e103649, DOI 10.15252/embj.2019103649 Link

Flp-in TREx 293

 Human embryonic kidney cell line (Derivat of HEK293) Human urinary system cell line adherent plasmid CRISPR Metafectene PRO publication B. K. Shrestha et al., J. Biol. Chem., 2019,doi/10.1074/jbc.RA119.010068 Link

Flp-in TREx 293

 Human embryonic kidney cell line (derivative of HEK293) Human urinary system cell line adherent plasmid CRISPR Metafectene PRO publication B.K. Shrestha et al., J Biol Chem., 2020, 295(5): 1240–1260, doi: 10.1074/jbc.RA119.010068 Link

Flp-In TREx HeLa

 Human cervix adenocarcinoma cell line (derivative of HeLa) Human genital tract cell line adherent plasmid CRISPR Metafectene PRO publication T. M. Nthiga et al., The EMBO J., 39: e103649, DOI 10.15252/embj.2019103649 Link

HCT116

 Human colon adenocarcinoma cell line ATCC CCL-247 Human digestive organs cell line adherent plasmid CRISPR Metafectene PRO publication J. Galán‐Martínez et al., Mol. Oncology, 2021, doi:10.1002/1878-0261.13085 Link

HCT116 p53 -/-

 Human colon adenocarcinoma cell line deficient of p53 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

HCT116 p53 -/-

 Human colon adenocarcinoma cell line deficient of p53 Human digestive organs cell line adherent plasmid CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

HECa10

 Mouse endothelial cells of peripheral lymph nodes Mouse immune system cell line adherent plasmid CRISPR K2 Transfection System publication P. Janus et al., Cell Death & Differentiation, 2020, 27: 2280–2292, doi.org/10.1038/s41418-020-0501-8 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR K2 Transfection System publication R. Roman et al., BMC Proceedings, 2020, 14 (Suppl 5): P-112 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR Metafectene publication J. Ohnheiser et al., Biochim. et Biophys. Acta, 2015, 1853: 1564-1573; doi.org/10.1016/j.bbamcr.2015.03.008 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR Metafectene PRO publication D. P. Reguera et al., Cells, 2020, 9: 443, doi:10.3390/cells9020443 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent plasmid CRISPR Metafectene publication A. de Araujo Brasil et al., PNAS, 2019, 116, 51: 25991–26000, doi/10.1073/pnas.1902483116 Link

HEK293

 Human embryonic kidney cell line ATCC CRL-1573 Human urinary system cell line adherent CRISPR Metafectene PRO publication S. Kumar et al., Dev. Cell., 2019, 49(1): 130–144.e6., doi:10.1016/j.devcel.2019.01.027. Link

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