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Products Transfection
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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein Mouse unknown unknown siRNA RNA-Interference Metafectene publication S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 Link

Mouse (nude) xenografted tumor, established by human Hep-2 cells Mouse unknown unknown plasmid RNA-Interference Metafectene publication J. Zhou et al., Lin Chuang Er Bi Yan Hou Ke Za Zhi, Mar 2006; 20(6): 264-7

Rat sensory neurons isolated from dorsal root ganglia (DRG) Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335 Link

Human primary fibroblasts (derived from ALS patients) Human unknown cell line adherent siRNA RNA-Interference Metafectene publication M. Milani et al., J. of Neuroinflammation, 2021, 18: 132, doi.org/10.1186/s12974-021-02184-1 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748 Link

Human keratinocytes isolated from neonatal foreskin Human skin primary cell adherent siRNA RNA-Interference Metafectene publication S. Bruche, PhD Thesis, 2014, Imperial College London Link

Hamster fetal ovary (organ culture) Hamster genital tract organ siRNA RNA-Interference Metafectene publication S. K. Roy et al., Methods and Protocols, 825, DOI 10.1007/978-1-61779-436-0_12

Mouse primary cortical cells Mouse brain primary cell adherent siRNA RNA-Interference Metafectene publication B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340

Human primary chronic lymphocytic leukemia cells Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

Mouse (18.5 dpc fetal or neonatal) ovaries Mouse genital tract unknown siRNA RNA-Interference Metafectene publication H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54 Link

Rabbit (New Zealand) primary chondrocytes from articular cartilage Rabbit other primary cell adherent siRNA RNA-Interference Metafectene publication J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047 Link

Human primary skin fibroblasts Human skin primary cell adherent siRNA RNA-Interference Metafectene publication M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1 Link

Bovine granulosa cells isolated from follicles (2-8 mm in diameter) of bovine ovaries Cow genital tract primary cell siRNA RNA-Interference Metafectene publication M. R. Plewes et al., bioRxiv, 2018, doi.org/10.1101/406256 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529 Link

Human primary fibroblasts Human unknown primary cell siRNA RNA-Interference Metafectene publication L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997 Link

Rat ventricular myocytes Rat muscle primary cell adherent plasmid RNA-Interference Metafectene publication B. Hu et al., BioScience Trends., 2012; 6(1): 26-32, DOI: 10.5582/bst.2012.v6.1.26 Link

Rat (Wistar neonatal) primary hippocampal neurons Rat brain primary cell adherent siRNA RNA-Interference Metafectene publication P. J. Kammermeier, J. Neurosci., Aug 2008; 28: 8560 - 8567 Link

Rabbit (New Zealand) primary chondrocytes from articular cartilage Rabbit other primary cell adherent siRNA RNA-Interference Metafectene publication E.-H. Hong et al., J. Biol. Chem., Aug 2011; 286: 28619 - 28631 Link

Rat (newborn) ovary cells Rat genital tract unknown siRNA RNA-Interference Metafectene publication L. Q. Xu et al., Sheng Li Xue Bao, Oct 2009; 61(5): 424-30

Mouse cultured fetal ovaries Mouse genital tract primary cell siRNA RNA-Interference Metafectene publication B. Xu et al., PLoS One, 2011, 6(1): e16046, doi: 10.1371/journal.pone.0016046 Link

1BR3hTERT

Human hTERT immortalized fibroblasts Human unknown unknown adherent siRNA RNA-Interference Metafectene publication T. Stiff et al., Hum. Mol. Genet., Apr 2016; 25: 1574 - 1587 Link

293-TLR3

Human embryonic kidney cell line stably expressing TLR3 (derivat of HEK293) Human urinary system cell line adherent plasmid RNA-Interference Metafectene publication R. Higgs et al., J. Immunol., Aug 2008; 181: 1780 - 1786 Link

293-TLR4/MD2

Human embryonic kidney cell line stably expressing TLR4/MD2 (derivat of HEK293) Human urinary system cell line adherent plasmid RNA-Interference Metafectene publication R. Higgs et al., J. Immunol., Aug 2008; 181: 1780 - 1786 Link

293A

Human embryonic kidney adenovirus producer cell line (derivat of HEK293) DSM ACC 305 Human urinary system cell line adherent siRNA RNA-Interference Metafectene publication W. Kim et al., J. Biol. Chem., 287 (8): 5278–5289, DOI 10.1074/jbc.M111.281709 Link

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