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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Plutella xylostella (hemocoels of third-instar NIL-R larvae, diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication Z. Guo et al., PLoS Pathog., 2021, 17(9): e1009917, doi.org/10.1371/journal.ppat.1009917 Link

Rat (Wistar neonatal) primary hippocampal neurons Rat brain primary cell adherent siRNA RNA-Interference Metafectene publication P. J. Kammermeier, J. Neurosci., Aug 2008; 28: 8560 - 8567 Link

Mouse hepatocytes Mouse digestive organs primary cell adherent plasmid Metafectene PRO publication S.-Y. Cai et al., JCI Insight. 2017, 2(5): e90780, doi.org/10.1172/jci.insight.90780

Human cutaneous melanoma (FFPE tissue sections) Human skin primary cell adherent plasmid Metafectene PRO publication M. Venza et al., Biochim. et Biophys. Acta, 2015, 1849: 247-256; doi.org/10.1016/j.bbagrm.2014.12.004

4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein Mouse unknown unknown siRNA RNA-Interference Metafectene publication S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 Link

Plutella xylostella (1 day old L4 larvae, diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M.C. Roy et al., Arch. Insect Biochem. Physiol., 2020, 104: e21670, DOI: 10.1002/arch.21670

Mouse podocytes Mouse urinary system primary cell adherent siRNA RNA-Interference K2 Transfection System publication F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4 Link

Plutella xylostella early third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Z. Guo et al., 2015, PLoS Genet 11(4): e1005124. doi:10.1371/journal.pgen.1005124 Link

Plodia interpunctella larvae (Indian meal moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication G. Deok Han et al., Pesticide Biochem. and Physiol., Nov. 2017, 143: 48-56, doi.org/10.1016/j.pestbp.2017.09.010

European Corn Borer (ECB) Larvae Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. M. W. Cooper, Dissertation, 2020, Mississippi State University Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent plasmid Metafectene PRO publication J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8

Maruca vitrata late L4 larvae (legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. Al Baki et al., Pest Manag. Sci., 2020, 76: 1020–1030, DOI: 10.1002/ps.5612 Link

Maruca vitrata hemocoel of L5D1 larvae (legume pod borer) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. A. Al Baki et al., PLoS ONE, 2018, 13 (10): e0204935, doi.org/10.1371/journal. pone.0204935 Link

Human keratinocytes isolated from neonatal foreskin Human skin primary cell adherent siRNA RNA-Interference Metafectene publication S. Bruche, PhD Thesis, 2014, Imperial College London Link

Plutella xylostella (hemocoels of early third-instar DBM1Ac-S larvae, diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication J. Zhou et al., Pest Manag Sci, 2020, 76: 712–720, DOI 10.1002/ps.5569

4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein Mouse unknown unknown siRNA in vivo application Metafectene publication S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 Link

Mouse cultured hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg

Plutella xylostella hemocoel of late second instar larvae Insect unknown unknown dsRNA in vivo application Metafectene PRO publication S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017

Aedes albopictus (mosquito, 5 days old females) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication Md. A. Al Baki et al., bioRxiv, 2020, doi.org/10.1101/2020.07.17.208389 Link

Tribolium castaneum (Hemocoel of late-instar larvae) Insect unknown unknown plasmid Metafectene PRO publication R. Hepat et al., J. Virol., October 2013, Volume 87, Number 20, 11223–11230 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent plasmid Metafectene PRO publication A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212

Chicken (Brown Leghorn) embryos Chicken unknown unknown plasmid Metafectene PRO application note

Maruca vitrata hemocoel of L5 larvae (legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. Al Baki et al., Arch. Insect Biochem. Physiol., 2019, 100: e21524, DOI: 10.1002/arch.21524 Link

Plutella xylostella, third instar larvae (diamondback or cabbage moth) Insect unknown unknown plasmid in vivo application Metafectene PRO publication B. Park et al., Insect Science, 2012, 19: 47–54, DOI 10.1111/j.1744-7917.2011.01421.x

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication M. C. Roy et al., Front. Immunol., 2021, 2: 791319, doi: 10.3389/fimmu.2021.791319 Link

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