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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Human fetal cortical stem cell cultures Human brain stem cell adherent plasmid Metafectene publication S. Couillard-Despres et al., 2014, US 8,841,430 Link

Neurosphere derived neuroprogenitor cells (isolated from newborn mice brains) Mouse brain unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System publication S.Y. Kim et al., J. of Cell Science, 2019, 132, jcs228940, doi:10.1242/jcs.228940 Link

Mouse cerebral cortical tissue (astrocytes) of 2-day-old SAMR1 and SAMP8 mice Mouse brain primary cell adherent siRNA Metafectene PRO publication M .J. Alvarez-López et al., J. of Neuroinflammation, 2014, 11:126, doi:10.1186/1742-2094-11-126 Link

Mouse primary embryonic forebrain cells Mouse brain primary cell plasmid Metafectene publication C. Karl et al., J Neurochemistry, 2005, 92, 264-282

Mouse mixed neuron-glia cerebellar cultures Mouse brain unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System application note

Rat (Wistar embryonic) primary hippocampal neurons Rat brain primary cell adherent plasmid Metafectene PRO publication K. Kankowski, Dissertation, 2015, Universitätsklinikum Hamburg-Eppendorf Link

Mouse cultured cortical neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg

Rat (Wistar neonatal) primary hippocampal neurons Rat brain primary cell adherent siRNA RNA-Interference Metafectene publication P. J. Kammermeier, J. Neurosci., Aug 2008; 28: 8560 - 8567 Link

Mouse hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R.C. Weethmann et al., Neuropharmacology, 2020, doi.org/10.1016/j.neuropharm.2020.108426 Link

Mouse primary cortical cells Mouse brain primary cell adherent siRNA RNA-Interference Metafectene publication B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340

Mouse cortical neuronal cells Mouse brain unknown adherent plasmid Metafectene EASY application note

Mouse embryonic forebrain cultures Mouse brain primary cell adherent plasmid Metafectene publication S. Couillard-Despres et al., 2014, US 8,841,430 Link

Mouse primary hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R. C.Werthmann et al., Neuropharmacology, 2021, 190: 108426, doi.org/10.1016/j.neuropharm.2020.108426 Link

Chicken cerebellar granule neurons Chicken brain primary cell adherent plasmid Metafectene PRO publication L. P. E. Austdal et al., Journal of Neuroendocrinology, 2016, 28, doi: 10.1111/jne.1243

Chicken cerebellar granule neurons Chicken brain unknown adherent plasmid Metafectene PRO publication B. O. Strom et al., J Neurosci Methods, Oct 2010; 193(1): 39-46

Mouse cultured hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg

Rat primary pinealocyte cells Rat brain primary cell plasmid Metafectene publication T.-D. Kim et al.,Genes & Dev., Apr 2007; 21: 797 - 810 Link

Rat embryonic cerebral cortex Rat brain unknown plasmid Metafectene PRO application note

A172

 Human glioma cells ATCC CRL-1620 Human brain unknown adherent plasmid Metafectene PRO application note

A172

 Human glioma cells ATCC CRL-1620 Human brain unknown adherent siRNA RNA-Interference Metafectene PRO publication M.C. Burger et al., Int. J. of Oncology, 2012, 41: 235-241, DOI: 10.3892/ijo.2012.1446 Link

AtT20

 Mouse pituitary tumor cell line ATCC CCL-89 Mouse brain cell line plasmid Metafectene publication T. Takahashi et al., J. Biochem., Nov 2009; 146: 675 - 682

BE(2)M17

 Human neuroblastoma cells ATCC CRL-2267 Human brain unknown adherent plasmid Metafectene PRO application note

BTEC

 Human brain glioblastoma endothelial cells Human brain unknown plasmid Metafectene PRO publication G. Tabatabai et al., Brain, Oct 2008; 131: 2579 - 2595 Link

BV-2

 Mouse immortalized microglial cell line Mouse brain cell line adherent plasmid Metafectene publication D.-Y. Kim et al., J. Neuroimmun. Pharmacol., 2021, 16: 470–482, doi.org/10.1007/s11481-020-09943-6 Link

BV-2

 Mouse microglial cell line Mouse brain cell line adherent plasmid Metafectene publication G. Lee et al., Phytomedicine, 2019, 55: 50–57, doi.org/10.1016/j.phymed.2018.06.032 Link

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