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References

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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

293A

Human embryonic kidney adenovirus producer cell line (derivat of HEK293) DSM ACC 305 Human urinary system cell line adherent sgRNA CRISPR Metafectene SI publication K. Kang et al., J. of Animal Sci. and Biotech., 2019, doi.org/10.1186/s40104-019-0354-510: 43 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent sgRNA CRISPR K4 Transfection System publication D. Marks et al., bioRxiv preprint, 2021, doi.org/10.1101/2021.07.01.450700 Link

6E4 HeLaT

Human cervix adenocarcinoma cell line (derivative of HeLa) Human genital tract cell line adherent sgRNA CRISPR Metafectene publication D. Kim et al., Nat ure Struct ural & Molec ular Biology, 2020, 27: 581–588, doi.org/10.1038/s41594-020-0427-3

6E4 HeLaT

Human cervix adenocarcinoma cell line (derivative of HeLa) Human genital tract cell line adherent sgRNA cotransfection (plasmid/sgRNA) Metafectene publication D. Kim et al., Nat ure Struct ural & Molec ular Biology, 2020, 27: 581–588, doi.org/10.1038/s41594-020-0427-3

HCT116 p53 -/-

Human colon adenocarcinoma cell line deficient of p53 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

Hep-3B

Human hepatocellular carcinoma cell line ATCC HB-8064 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication R. Han et al., Scientific Reports, 2021, 11: 8626, doi.org/10.1038/s41598- Link

MIA PaCa-2

Human pancreas carcinoma cell line ATCC CRL-1420 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

NCI-H1299

Human lung carcinoma cell line ATCC CRL-5803 Human respiratory system cell line adherent sgRNA CRISPR Metafectene PRO publication C. Ren et al., J. Biol. Chem., Apr 2016; 291: 9629 - 9637 Link

SNU-449

Human hepatocellular carcinoma cell line ATCC CRL-2234 Human digestive organs cell line adherent sgRNA CRISPR Metafectene PRO publication R. Han et al., Scientific Reports, 2021, 11: 8626, doi.org/10.1038/s41598- Link

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