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µ-Transfection Kit VI from Biontex for high resolution live cell imaging

µ-Transfection Kit VI

Cell culture, transfection and high-resolution microscopy directly in the culture vessel
Product Information

Highlights:

  • Simple transfection protocol (no optimization necessary)
  • DNA and siRNA transfection
  • Immediate live observation possible
  • Ideal optical properties for transmitted light and fluorescence microscopy
  • Cells can be fixed and dyed
  • Compatible with oil immersion

The μ-Transfection Kit VI is a unique combination of two elements tailored to each other in a highperformance kit permitting high-resolution microscopy of living transfected cells. The joint kit comprises μ-Slides VI from ibidi and the reagent METAFECTENE® μ, developed by Biontex especially for this purpose.

The kit enables cell transfection to be performed with both DNA and siRNA. The optimization procedure usually necessary in plasmid transfection is eliminated, while in siRNA transfection optimum knockdown is achieved with only three differing lipoplex volumes.

Content of the μ-Transfection Kit VI

The ultra-simple protocol provides for treatment of a cell suspension with lipoplexes and transfer directly onto the μ-Slides. The resulting effects can be subsequently observed in the living cells in outstanding optical quality (live cell imaging). Examples of applications are tracking the expression and path of a fluorescence-tagged protein through the cell or monitoring the effect of knockdown of a gene.

High-resolution images of transfection of HepG2 cells with pCMV-GFP using μ-Transfection Kit VI

High-resolution images of transfection of HepG2 cells with pCMV-GFP using μ-Transfection Kit VI (60× oil-immersion objective)

High-resolution images of transfection of HeLa cells with pCMV-GFP using μ-Transfection Kit VI

High-resolution images of transfection of HeLa cells with pCMV-GFP using μ-Transfection Kit VI (20× objective)

 

Product Specification

Application Transfection and high-resolution microscopy of living mammalian cells (live cell imaging)
Content METAFECTENE® µ, 1 x PBS, µ-Slides VI (6 channels each)
Assays 90 per kit
Shipping At room temperature
Storage ≤ –15°C METAFECTENE® μ; 4°C PBS
Shelf life 1 year (after date of delivery)
Product Group Microfection

K010-0.1

µ-Transfection Kit VI
100 μl METAFECTENE® μ
10 ml 1 x PBS
15 Slides
Transfection kit for microscopy

€345.00