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Proteofection reagent PROTEOfectene® from Biontex

PROTEOfectene®

High-efficiency proteofection reagent for mammalian cells
Product Information

Highlights:

  • High efficiency
  • Broad range of application with both proteins and cells
  • Ultra-low toxicity – cell activity is maintained
  • Full serum compatibility
  • Efficient and rapid protocol: proteofection within 6 hours

 

PROTEOfectene® enables proteins to be introduced directly into mammalian cells without the need for prior gene expression.

Proteins introduced with PROTEOfectene® retain their structure and function completely unchanged. This opens new possibilities, from localization studies using fluorescence-marked proteins to direct manipulation of cell metabolism by means of functionally capable enzymes.

Some major features of PROTEOfectene® are its high efficiency, low toxicity and serum compatibility, making it a unique addition to the range of protein transport reagents. R-Phycoerythrin is a very suitable red fluorescent protein for application as positive control in proteofection experiments.

Proteofection of CHO-K1 cells with β-galactosidase and PROTEOfectene®

Proteofection of CHO-K1 cells with β-galactosidase and PROTEOfectene®. The subsequently fixed and dyed slide shows the functional capability of the enzyme introduced.

Proteofection of BEAS-2B cells with R-phycoerythrin and PROTEOfectene®

Proteofection of BEAS-2B cells with R-phycoerythrin and PROTEOfectene®.

 

 

Proteofection of Hep G2 cells with R-Phycoerythrin

Proteofection of Hep G2 cells by using the µ-Proteofection Kit VI. Pictures were recorded 24h after addition of the PROTEOfectene/R-Phycoerythrin complex:

Light image after proteofection of HepG2 cells with R-phycoerythrin Fluorescence image after proteofection of HepG2 cells with R-phycoerythrin

Picture of cells

Fluorescence picture: Most bright spots are cell fragments loaded with R-Phycoerythrin. Note the fluorescent cytoplasmas and nonluminous cell nuclei.

 

Proteofection of HeLa cells with GST-NLS-GFP protein:

Proteofection of HeLa cells by using µ-Proteofection Kit VI. Pictures were recorded 24h after addition of the PROTEOfectene®/GST-NLS-GFP complex:

Light image after proteofection of HeLa cells with GST-NLS-GFP protein Fluorescence image after proteofection of HeLa cells with GST-NLS-GFP protein

Picture of cells

Fluorescence picture. Move cursor over the picture for viewing the overlay picture: Cell nuclei have quantitatively imported the NLS-GFP protein.

 

 

Product Specification

Application Proteofection of mammalian cells with proteins
Content PROTEOfectene®, 100 µl R-Phycoerythrin (100 µg/ml) as positive control
Assays Approx. 100 (24-well) or approx. 20 (6-well) per 100 µl reagent
Shipping At room temperature
Storage 4°C
Shelf life 1/2 year (from date of delivery)
Product Group Proteofection

E010-0.1

PROTEOfectene® Kit
100 μl Proteofection reagent
100 μl R-Phycoerythrin
Transfection kit for proteins

€172.00
E010-0.25

PROTEOfectene® Kit
250 μl Proteofection reagent
100 μl R-Phycoerythrin
Transfection kit for proteins

€350.00