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Proteofection reagent for antibodies PROTEOfectene® AB from Biontex

PROTEOfectene® AB - Proteofection Reagent for Antibodies

High-efficiency antibody proteofection reagent for mammalian cells

Product Information
Background Information
FAQ Proteofection

Highlights:

  • High efficiency and broad range of cell types
  • Mono- and polyclonal antibodies of all isotypes
  • Ultra-low toxicity – cell vitality is maintained
  • Full serum compatibility
  • Efficient, rapid protocol

PROTEOfectene® AB complexates antibodies noncovalently by means of electrostatic and hydrophobic interactions, forming a proteoplex which is taken up by cells through endocytosis and finally releases the antibodies in the cell cytosol while retaining full functionality and maintaining epitopic binding affinity. PROTEOfectene® AB can be used to introduce mono- and polyclonal antibodies of all isotypes.

Used with the incredible range of antibodies on the market, PROTEOfectene® AB creates new possibilities for research and manipulation of cell processes. For example, fluorescence-marked antibodies can be used for immunostaining in living cells, enabling live cell imaging of processes to be taken. By using antibodies against a variety of cell constituents – proteins/enzymes, transmitter substances, educts and products of metabolism – antibody proteofection becomes a method offering many possibilities.

Proteofection of A549 cells with PROTEOfectene® AB and AlexaFluor®488 labeled antibodies

Proteofection of A549 cells with PROTEOfectene® AB and AlexaFluor®488 labeled antibodies directed against the nuclear pore complex. Fluorescent microscopy clearly shows the selective binding of antibodies to nuclear pores.

Proteofection of A549 cells with AlexaFluor®488 labeled antibodies against giantin – a membrane protein of the Golgi apparatus – using PROTEOfectene® AB

Proteofection of A549 cells with AlexaFluor®488 labeled antibodies against giantin – a membrane protein of the Golgi apparatus – using PROTEOfectene® AB. Fluorescent microscopy clearly shows the selective binding of antibodies in the region of the Golgi apparatus.

 

 

Product Specification

Application Proteofection of eukaryotic cells with anti bodies
Content PROTEOfectene® AB, 100 µl IgG (FITC labeled; 100 µg/ml) as positive control
Assays Approx. 100 (24-well) or approx. 20 (6-well) per 100 µl reagent
Shipping At room temperature
Storage 4°C PROTEOfectene®AB;
≤ –15°C IgG
Shelf life 1/2 year (after date of delivery)
Product Group Proteofection

E020-0.1

PROTEOfectene® AB Kit
100 μl Antibody proteofection reagent
100 μl IgG-FITC (100μg/ml)
Transfection kit for antibodies

€172.00
E020-0.25

PROTEOfectene® AB Kit
250 μl Antibody proteofection reagent
100 μl IgG-FITC (100μg/ml)
Transfection kit for antibodies

€350.00