Common transfection reagents require considerable efforts to optimize the DNA:lipid ratio and the amount of DNA used and, as a rule, involve considerable expenditure of time – 4 days – from seeding the cells to reporter gene assay. The idea behind the development of METAFECTENE® EASY⁺ was to establish an efficient transfection reagent combined with a fast and easy protocol.

METAFECTENE® EASY⁺ works with two predefined DNA/lipid ratios and a predefined amount of lipoplexes combined with a "Fast Forward" protocol. This enables high transfection efficiencies to be reached for a variety of robust mammalian cell lines and primary cells while minimizing optimization effort.

Main advantages of the "Fast Forward" protocol:

Using the "Fast Forward" protocol, cells are seeded and subsequently transfected without being allowed to grow on the vessel surface by incubation overnight. This results in a time saving of 24 hours, allowing two successive result-dependent transfections to be performed within a single working week.