METAFECTENE® SI⁺ consists of a lipid blend designed and optimized especially for siRNA (gene silencing by RNA interference/RNAi) and miRNA transfection. However, other nucleic acids can also be used.
Unlike for conventional DNA transfection, transport of the nucleic acid into the cell nucleus is not necessary for RNA transfection. The development of METAFECTENE® SI⁺ was thus primarily aimed at maximizing the endocytosis volume of the lipoplex plus ensuring rapid and complete release of RNA in the cytosol.
High knockdown rates with low RNA and reagent amounts combined with low toxicity make METAFECTENE® SI⁺ an ideal tool to be used for all mammalian cell lines and primary cells.
Comparison of knock-down of luciferase expression of cells stably expressing luciferase following transfection of anti-Luc-siRNA with METAFECTENE® SI⁺ or a commercial transfection reagent (L2k) under optimized conditions:
Main advantages of the "Fast Forward" protocol:
Using the "Fast Forward" protocol, cells are seeded and subsequently transfected without being allowed to grow on the vessel surface by incubation overnight. This results in a time saving of 24 hours, allowing two successive result-dependent transfections to be performed within a single working week.