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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Mouse primary hepatocytes Mouse digestive organs primary cell adherent plasmid cotransfection (plasmid/miRNA) Metafectene PRO publication S. Surendran et al., Scientific Reports, 2016, 6:18958; DOI: 10.1038/srep18958 Link

Bovine granulosa cells isolated from follicles (2-8 mm in diameter) of bovine ovaries Cow genital tract primary cell siRNA RNA-Interference Metafectene publication M. R. Plewes et al., bioRxiv, 2018, doi.org/10.1101/406256 Link

Plodia interpunctella fifth-instar larva (Indian meal moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. Kim et al., Environmental Entomology, 2017, 46 (4): 1005-1011, doi: 10.1093/ee/nvx112

Spodoptera exigua third and fifth instar larvae (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication H. S. Kim et al., Journal of Asia-Pacific Entomology, March 2017, 20 (1): 199-205

Rat sensory neurons isolated from dorsal root ganglia (DRG) Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 Link

Mouse primary myoblasts Mouse unknown primary cell adherent plasmid Metafectene PRO publication C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814 Link

Mouse cultured cortical neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg

Plutella xylostella hemocoel of late second instar larvae Insect unknown unknown dsRNA cotransfection (plasmid/dsRNA) Metafectene PRO publication S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017

Maruca vitrata hemocoel of L5D1 larvae (legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication M. A. Al Baki et al., PLoS ONE, 2018, 13 (10): e0204935, doi.org/10.1371/journal. pone.0204935 Link

Spodoptera exigua (larval hemocoel) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Y. Park et al., J. Exp. Biol., Nov 2013; 216: 4196 - 4203 Link

Rabbit (New Zealand) primary chondrocytes from articular cartilage Rabbit other primary cell adherent siRNA RNA-Interference Metafectene publication J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047 Link

Human Praeadipocytes Human skin primary cell plasmid Metafectene publication G. Weisinger et al., US9663790B2 Link

Plutella xylostella third stage ABM-R larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication X. Gao et al., Int. J. Mol. Sci., 2016, 17: 274; doi:10.3390/ijms17030274 Link

Spodoptera exigua fifth instar larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication J. Park et al., Archives of Insect Biochem. and Physiol., 2014, 85(4) :234-247, DOI: 10.1002/arch.21156

Spodoptera exigua fifth instar larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication J. Park et al., PLoS ONE, 2014, 9(9): e105717, doi:10.1371/journal.pone.0105717 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212

Rabbit (New Zealand) primary chondrocytes from articular cartilage Rabbit other primary cell adherent plasmid Metafectene publication J. Rhee et al., J. Biol. Chem., Apr 2012; 287: 11751 - 11760 Link

Mouse fibroblast cell line, deficient of N-WASP Mouse unknown cell line adherent plasmid Metafectene publication S. Benesch et al., J. Cell Sci., Jul 2005; 118: 3103 - 3115 Link

Antheraea pernyi pupae in diaupase (Chinese Tasar Moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. A. M. Mohamed et al., PLoS ONE, 2014, 9(3): e92680, doi:10.1371/journal.pone.0092680 Link

Mouse immortalized skin fibroblasts Mouse unknown unknown adherent plasmid Metafectene PRO publication E. Bartova et al., Protoplasma, 2017, 254: 2035-2043, DOI 10.1007/s00709-017-1076-1

Plutella xylostella larval hemocoel (diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication X. Gu et al., Journal of Insect Physiology, 2015, 80: 81-87; doi.org/10.1016/j.jinsphys.2015.02.001

Rabbit primary fibroblasts Rabbit unknown primary cell adherent plasmid stable transfection Metafectene EASY application note

Human fibrous dysplasia cells Human unknown unknown plasmid Metafectene publication P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44

Maruca vitrata hemocoel of L5 larvae (legume pod borer) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication A. A. Baki et al., Arch. Insect Biochem. Physiol., 2019:100:e21524, doi.org/10.1002/arch.21524

Spodoptera exigua third instar larvae (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication J.-B. Lee et al., Arch. Insect Biochem. Physiol., 2017, 94: e21371, DOI: 10.1002/arch.21371

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