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Transfection reagent METAFECTENE® PRO from Biontex

METAFECTENE® PRO

A highly efficient transfection reagent for mammalian cells
Product Information

Highlights:

  • Suitable for DNA, mRNA, siRNA and miRNA transfection as well as cotransfection of DNA and RNA
  • Minimized toxic effects
  • Optimized transfection rates
  • Free from serum inhibition
  • Comprehensive library of user reports
  • Wide range of references in specialist literature

Light image, fluorescence image and superimposed image after transfection of 293T cells using METAFECTENE PRO and pCMV-GFP

Transfection of 293T cells with pCMV-GFP and METAFECTENE® PRO

METAFECTENE® PRO is a liposome based transfection reagent consisting of a mixture of polycationic and neutral lipids. It is an advanced product based on METAFECTENE. Structural changes of the comprising cationic lipids results in higher efficiency and lower toxicity.

Low cytotoxicity and lack of serum inhibition allow for a wide range of applications. Metafectene® Pro is suitable for transient and stable transfection of mammalian cell lines and primary cells with DNA (plasmids, bacmids), RNA (mRNA, miRNA and siRNA) and modified nucleic acids (antisense oligonucleotides). Areas of application are particularly the production of proteins, antibodies, and viruses (e.g. adenoviruses, AAV, lentiviruses), cotransfections of different nucleic acids, genome editing (e.g. CRISPR/Cas9, CRISPR/Cpf1) and gene silencing (gene knockdown).

 

Transfection efficiency of METAFECTENE PRO and L2k after transfecting different cells with pCMV-Luc

 

Comparison of METAFECTENE® PRO with another commercial available Transfection reagent (L2K) in an transfection assay with pCMV-Luc under optimized conditions.

 

Product Specification

Application A highly efficient transfection reagent for mammalian cells
Content Cationic lipids and colipids in water
Assays Up to 1500 (24-well) or up to 400 (6-well) per 1 ml reagent
Shipping At room temperature
Storage 4°C
Shelf life 1 year (from date of delivery)
Product Group Transfection

T040-1.0

METAFECTENE® PRO 1 ml
Transfection reagent for mammalian cells

€205.00
T040-2.0

METAFECTENE® PRO 2 x 1 ml
Transfection reagent for mammalian cells

€405.00
T040-5.0

METAFECTENE® PRO 5 x 1 ml
Transfection reagent for mammalian cells

€925.00