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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent plasmid K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Rat (Sprague-Dawley) primary alveolar macrophages Rat immune system primary cell plasmid Metafectene publication M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 Link

Rat primary neonatal ventricular cardiomyocytes Rat muscle primary cell plasmid K2 Transfection System application note

Rat primary skeletal myoblast cells (from male newborn Lewis rats) Rat muscle primary cell adherent plasmid Metafectene PRO publication B. Blumenthal et al., Eur. J. Cardio-thoracic Surg., 2011, 40: e135—e141, doi:10.1016/j.ejcts.2011.05.026 Link

Primary rat neuronal dendrites Rat nervous system primary cell adherent mRNA Metafectene publication J. Eberwine et al., 2013, US 8,367.402

Rat primary pinealocyte cells Rat brain primary cell plasmid Metafectene publication T.-D. Kim et al.,Genes & Dev., Apr 2007; 21: 797 - 810 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335 Link

Rat primary granulosa cells isolated from Ovaries Rat genital tract primary cell plasmid Metafectene publication J.-H. Kim et al., Nature Commun., 2014, 5:2936, DOI: 10.1038/ncomms3936 Link

Rat (Sprague-Dawley) Rat unknown unknown plasmid in vivo application Metafectene publication T. Holzbach et al., J Cell Mol Med, Mar 2010; 14(3): 587-99

Rat primary hepatocytes (Sprague Dawley rats) Rat digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991 Link

Rat (Wistar neonatal) primary hippocampal neurons Rat brain primary cell adherent siRNA RNA-Interference Metafectene publication P. J. Kammermeier, J. Neurosci., Aug 2008; 28: 8560 - 8567 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529 Link

Rat ventricular myocytes Rat muscle primary cell adherent plasmid RNA-Interference Metafectene publication B. Hu et al., BioScience Trends., 2012; 6(1): 26-32, DOI: 10.5582/bst.2012.v6.1.26 Link

Rat sensory neurons isolated from dorsal root ganglia (DRG) Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 Link

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent siRNA RNA-Interference K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Rat (Wistar adult male) hepatocytes Rat digestive organs unknown adherent plasmid DOTAP publication E. Skarpen et al., FASEB J, Feb 2008; 22: 466 - 476 Link

Rat primary hepatocytes Rat digestive organs primary cell adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

Rat embryonic cerebral cortex Rat brain unknown plasmid Metafectene PRO application note

Rat (newborn) ovary cells Rat genital tract unknown siRNA RNA-Interference Metafectene publication L. Q. Xu et al., Sheng Li Xue Bao, Oct 2009; 61(5): 424-30

Rat primary hepatocytes Rat digestive organs primary cell adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748 Link

Rat (Wistar embryonic) primary hippocampal neurons Rat brain primary cell adherent plasmid Metafectene PRO publication K. Kankowski, Dissertation, 2015, Universitätsklinikum Hamburg-Eppendorf Link

A7

Rat transformed retinal astrcyte cell line Rat sensory organs cell line adherent plasmid K2 Transfection System publication D. W. Chen et al., Drug Deliv. and Transl. Res., DOI 10.1007/s13346-016-0324-9

A7

Rat transformed retinal astrcyte cell line Rat sensory organs cell line adherent plasmid K2 Transfection System application note

AR42J

Rat pancreas tumor cell line ATCC CRL-1492 Rat digestive organs cell line adherent plasmid Metafectene PRO publication C. Sæten Fjeldbo et al., Am J Physiol Gastrointest Liver Physiol 302: G21–G33, 2012 Link

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