References
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Instruction for Use:
In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.
No reference? > General Selection Guide
If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.
CELL TYPE | DESCRIPTION OF CELL TYPE | CAT.NO. | SPECIES | ORIGIN OF CELLS | COMMON CELL TYPE | GROWTH PROPERTIES | NUCLEIC ACID / DELIVERED MOLECULE | APPLICATION | PRODUCT | REFERENCE TYPE | BIBLIOGRAPHIC DATA | LINK | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | miRNA | Metafectene PRO | publication | C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental | |||||
Mouse primary myoblasts | Mouse | unknown | primary cell | adherent | miRNA | Metafectene PRO | publication | C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814 | Link | ||||
Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | miRNA | Metafectene PRO | publication | S. Surendran, PhD Thesis, 2014, Indiana University | |||||
Human primary hepatocytes | Human | digestive organs | primary cell | adherent | miRNA | Metafectene PRO | publication | J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 | Link | ||||
Mouse (18.5 dpc fetal or neonatal) ovaries | Mouse | genital tract | unknown | miRNA | Metafectene | publication | H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54 | Link | |||||
Human Primary Peripheral Blood CD14+ Monocytes | ATCC PCS-800-010 | Human | other | primary cell | suspension | miRNA | Metafectene SI | publication | S. Smith et al., Journal of Autoimmunity, 2017, 79, 105e111 | Link | |||
Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | miRNA | Metafectene PRO | publication | J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8 | |||||
Human primary CD14+ monocytes | Human | other | primary cell | suspension | miRNA | Metafectene SI | publication | S. Smith, JCI Insight, 2018, 3(15):e120798, doi.org/10.1172/jci.insight.120798 | Link | ||||
Mouse (BALB/c mice) organ of Corti explants | Mouse | sensory organs | unknown | miRNA | K2 Transfection System | publication | Y. Li et al., Cell Death and Disease, 2016, 7:e2362, doi:10.1038/cddis.2016.246 | Link | |||||
Human podocytes | Human | urinary system | unknown | adherent | miRNA | K2 Transfection System | application note | ||||||
Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | miRNA | cotransfection (plasmid/miRNA) | Metafectene PRO | publication | S. Surendran et al., Scientific Reports, 2016, 6:18958; DOI: 10.1038/srep18958 | Link | |||
293A |
Human embryonic kidney adenovirus producer cell line (derivat of HEK293) | Human | urinary system | cell line | adherent | miRNA | K2 Transfection System | publication | H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 | Link | |||
293FT |
Human embryonic kidney virus producer cell line (derivat of 293T) | Human | urinary system | cell line | adherent | miRNA | cotransfection (plasmid/miRNA) | Metafectene | publication | T.-D. Kim et al., Blood, Nov 2011; 118: 5476 - 5486 | Link | ||
293T |
Human embryonic kidney cell line, SV40 large T antigen inserted | ATCC CRL-3216 | Human | urinary system | cell line | adherent | miRNA | cotransfection (plasmid/miRNA) | Metafectene | publication | s. Guan, Dissertation , 2018, Ruprecht-Karls-Universität Heidelberg | ||
293T |
Human embryonic kidney cell line, SV40 large T antigen inserted | ATCC CRL-3216 | Human | urinary system | cell line | adherent | miRNA | cotransfection (plasmid/miRNA) | K2 Transfection System | publication | Z. Cai et al., Exp. & Mol. Med., 2018, 50:45, DOI 10.1038/s12276-018-0068-3 | Link | |
293T |
Human embryonic kidney cell line, SV40 large T antigen inserted | ATCC CRL-3216 | Human | urinary system | cell line | adherent | miRNA | RNA-Interference | K2 Transfection System | publication | V. Burghi et al., Front. Pharmacol., 2019, 10: 146, doi: 10.3389/fphar.2019.00146 | Link | |
293T |
Human embryonic kidney cell line, SV40 large T antigen inserted | ATCC CRL-3216 | Human | urinary system | cell line | adherent | miRNA | cotransfection (plasmid/miRNA) | Metafectene | publication | S. Miretti et al., BMC Genomics, 2013, 14: 194, doi:10.1186/1471-2164-14-194 | Link | |
3T3-L1 |
Mouse embryonic fibroblast cell line | ATCC CL-173 | Mouse | unknown | cell line | adherent | miRNA | Metafectene PRO | publication | C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental | |||
3T3-L1 |
Mouse embryonic fibroblast cell line | ATCC CL-173 | Mouse | unknown | cell line | adherent | miRNA | Metafectene PRO | publication | C. Castano et al., PNAS, 2018, 115(48):12158-12163 ,doi/10.1073/pnas.1808855115 | |||
C2C12 |
Mouse myoblast cell line | ATCC CRL-1772 | Mouse | muscle | cell line | adherent | miRNA | K2 Transfection System | publication | H. Qiu et al., Int. J. Biol. Sci. 2017, 13 (2): 157-166, doi: 10.7150/ijbs.17440 | Link | ||
C2C12 |
Mouse myoblast cell line | ATCC CRL-1772 | Mouse | muscle | cell line | adherent | miRNA | K2 Transfection System | publication | H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 | Link | ||
C2C12 |
Mouse myoblast cell line | ATCC CRL-1772 | Mouse | muscle | cell line | adherent | miRNA | Metafectene PRO | publication | C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814 | Link | ||
C2C12 |
Mouse myoblast cell line | ATCC CRL-1772 | Mouse | muscle | cell line | adherent | miRNA | Metafectene PRO | publication | C. E. Winbanks et al., PLoS ONE, 2013, 8(9 ): e73589, doi:10.1371/journal.pone.0073589 | Link | ||
C2C12 |
Mouse myoblast cell line | ATCC CRL-1772 | Mouse | muscle | cell line | adherent | miRNA | Metafectene PRO | publication | C. E. Winbanks et al., J. Biol. Chem., 286, 16: 13805–13814, DOI 10.1074/jbc.M110.192625 | Link | ||
CAFs |
Human cancer-associated fibroblasts (patient derived) | Human | unknown | primary cell | adherent | miRNA | Metafectene PRO | publication | G. T. Noh, Dissertation, 2021, The Graduate School Yonsei University | Link |