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References

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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Mouse primary hepatocytes Mouse digestive organs primary cell adherent miRNA Metafectene PRO publication C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental

Mouse primary myoblasts Mouse unknown primary cell adherent miRNA Metafectene PRO publication C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent miRNA Metafectene PRO publication S. Surendran, PhD Thesis, 2014, Indiana University

Human primary hepatocytes Human digestive organs primary cell adherent miRNA Metafectene PRO publication J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 Link

Mouse (18.5 dpc fetal or neonatal) ovaries Mouse genital tract unknown miRNA Metafectene publication H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54 Link

Human Primary Peripheral Blood CD14+ Monocytes ATCC PCS-800-010 Human other primary cell suspension miRNA Metafectene SI publication S. Smith et al., Journal of Autoimmunity, 2017, 79, 105e111 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent miRNA Metafectene PRO publication J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8

Human primary CD14+ monocytes Human other primary cell suspension miRNA Metafectene SI publication S. Smith, JCI Insight, 2018, 3(15):e120798, doi.org/10.1172/jci.insight.120798 Link

Mouse (BALB/c mice) organ of Corti explants Mouse sensory organs unknown miRNA K2 Transfection System publication Y. Li et al., Cell Death and Disease, 2016, 7:e2362, doi:10.1038/cddis.2016.246 Link

Human podocytes Human urinary system unknown adherent miRNA K2 Transfection System application note

Mouse primary hepatocytes Mouse digestive organs primary cell adherent miRNA cotransfection (plasmid/miRNA) Metafectene PRO publication S. Surendran et al., Scientific Reports, 2016, 6:18958; DOI: 10.1038/srep18958 Link

293A

Human embryonic kidney adenovirus producer cell line (derivat of HEK293) Human urinary system cell line adherent miRNA K2 Transfection System publication H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 Link

293FT

Human embryonic kidney virus producer cell line (derivat of 293T) Human urinary system cell line adherent miRNA cotransfection (plasmid/miRNA) Metafectene publication T.-D. Kim et al., Blood, Nov 2011; 118: 5476 - 5486 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent miRNA cotransfection (plasmid/miRNA) Metafectene publication s. Guan, Dissertation , 2018, Ruprecht-Karls-Universität Heidelberg

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent miRNA cotransfection (plasmid/miRNA) K2 Transfection System publication Z. Cai et al., Exp. & Mol. Med., 2018, 50:45, DOI 10.1038/s12276-018-0068-3 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent miRNA RNA-Interference K2 Transfection System publication V. Burghi et al., Front. Pharmacol., 2019, 10: 146, doi: 10.3389/fphar.2019.00146 Link

293T

Human embryonic kidney cell line, SV40 large T antigen inserted ATCC CRL-3216 Human urinary system cell line adherent miRNA cotransfection (plasmid/miRNA) Metafectene publication S. Miretti et al., BMC Genomics, 2013, 14: 194, doi:10.1186/1471-2164-14-194 Link

3T3-L1

Mouse embryonic fibroblast cell line ATCC CL-173 Mouse unknown cell line adherent miRNA Metafectene PRO publication C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental

3T3-L1

Mouse embryonic fibroblast cell line ATCC CL-173 Mouse unknown cell line adherent miRNA Metafectene PRO publication C. Castano et al., PNAS, 2018, 115(48):12158-12163 ,doi/10.1073/pnas.1808855115

C2C12

Mouse myoblast cell line ATCC CRL-1772 Mouse muscle cell line adherent miRNA K2 Transfection System publication H. Qiu et al., Int. J. Biol. Sci. 2017, 13 (2): 157-166, doi: 10.7150/ijbs.17440 Link

C2C12

Mouse myoblast cell line ATCC CRL-1772 Mouse muscle cell line adherent miRNA K2 Transfection System publication H. Qiu et al., Cell Death and Differentiation, 2016, 23: 1658-1669, doi:10.1038/cdd.2016.56 Link

C2C12

Mouse myoblast cell line ATCC CRL-1772 Mouse muscle cell line adherent miRNA Metafectene PRO publication C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814 Link

C2C12

Mouse myoblast cell line ATCC CRL-1772 Mouse muscle cell line adherent miRNA Metafectene PRO publication C. E. Winbanks et al., PLoS ONE, 2013, 8(9 ): e73589, doi:10.1371/journal.pone.0073589 Link

C2C12

Mouse myoblast cell line ATCC CRL-1772 Mouse muscle cell line adherent miRNA Metafectene PRO publication C. E. Winbanks et al., J. Biol. Chem., 286, 16: 13805–13814, DOI 10.1074/jbc.M110.192625 Link

CAFs

Human cancer-associated fibroblasts (patient derived) Human unknown primary cell adherent miRNA Metafectene PRO publication G. T. Noh, Dissertation, 2021, The Graduate School Yonsei University Link

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