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µ-Transfection Kit VI FluoR from Biontex for high resolution live cell imaging

µ-Transfection Kit VI FluoR

Visualizing lipofection
Product Information

Highlights:

  • Ideal optical properties for transmitted light and fluorescence microscopy
  • Visualization of transfection process in living cells
  • Simple transfection protocol (no optimization necessary)
  • DNA and siRNA transfection
  • Cells can be fixed and dyed
  • Compatible with oil immersion

High-resolution microscopic view of HepG2 cells transfected with the μ-Transfection Kit VI FluoR

μ-Transfection Kit VI FluoR is the ideal solution where visualization of the carrier system's path through the cell is necessary, for example to gain information about the transfection process.

In addition to all the benefits of the μ-Transfection Kit VI FluoR, this kit offers the option of tracking lipoplexes in living cells. For this purpose the transfection reagent METAFECTENE® μ was labeled with the fluorescent dye rhodamine as METAFECTENE® μ FluoR, while retaining its other properties. This enables the transfection process itself to be tracked microscopically.

High-resolution microscopic view of HepG2 cells transfected with the μ-Transfection Kit VI FluoR. The images clearly show the fluorescent red-marked lipoplexes taken up by endocytosis and the incipient expression of the fluorescent green GFP. (Images of the individual fluorescence channels and layered images).

 

 

HepG2 cells have been transfected with the plasmid pCMV-eGFP by use of the µ-Transfection Kit VI FluoR (METAFECTENE® µ FluoR, Biontex in µ-Slides VI0.4, ibidi). The film shows an overlay of phase contrast images of the cells combined with fluorescence images of rhodamine-labeled lipid (red channel) and expressed GFP (green channel). Recording began directly after plating the transfected cell suspension and ended 24 h later.

Product Specification

Application Visualization of lipofection by fluorescence and high resolution microscopy of living mammalian cells (live cell imaging)
Content METAFECTENE® µ FluoR
1 x PBS
µ-Slides VI (6 channels each)
Assays 90 per kit
Shipping At room temperature
Storage 4°C
Shelf life 1 year (after date of delivery)
Product Group Microfection

K020-0.1

µ-Transfection Kit VI FluoR
100μl METAFECTENE®μFluoR
10 ml 1 x PBS
15 Slides
Transfection kit for microscopy, fluorescence-labelled

$624.00